Browsing by Subject "Cellular proliferation"

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    Accelerated Simple Preparation of Curcumin-Loaded Silk Fibroin/Hyaluronic Acid Hydrogels for Biomedical Applications
    (MDPI, 2023-01-18) Chaala, M.; Seeba, F. Z.; Fuster, M. G.; Moulefera, I.; Montalbán, M. G.; Carissimi, G.; Víllora Cano, Gloria; Ingeniería Química; Facultad de Química
    The development of new biomaterials from natural fibres in the field of biomedicine have attracted great interest in recent years. One of the most studied fibres has been silk fibroin produced by the Bombyx mori worm, due to its excellent mechanical properties and its biodegradability and bioavailability. Among the different biomaterials that can be prepared from silk fibroin, hydrogels have attracted considerable attention due to their potential use in different fields, such as scaffolding, cell therapy and biomedical application. Hydrogels are essentially a three-dimensional network of flexible polymer chains that absorb considerable amounts of water and can be loaded with drugs and/or cells inside to be used in a wide variety of applications. Here we present a simple sonication process for the preparation of curcumin-hyaluronic acid-silk fibroin hydrogels. Different grades of hydrogels were prepared by controlling the relative amounts of their components. The hydrogels were physically and morphologically characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM) and their biological activity was tested in terms of cell viability in a fibroblast cell line.
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    Cellular proliferation in the rat pineal gland during postnatal development
    (Murcia : F. Hernández, 1998) Carvajal, J.C.; Carbajo, S.; Gómez Esteban, M.B.; Alvarez-Morujo, A.J.; Muñoz Barragan, L.
    To establish a possible correlation between the rate of cellular proliferation and already documented functional and morphological characteristics of the rat pineal gland during postnatal development, the bromodeoxyuridine labelling method was used to evaluate the fraction of cells at the S phase of the cell cycle in paraffin sections from I-, 7-, 14- and 28-day-old rats. Numerical density, taken as an indirect measure of cell hypertrophy, was also evaluated. During the first week after birth the percentage of S phase-cells in the rat pineal gland sharply decreased from around 9% to 1.3%. A smaller but also significant decrease was found from the 7th to the 14th postnatal day where S phase cells were less than 0.5% of all pineal cells. A very low percentage was also seen in samples from 28-day-old rats. Numerical density, namely, the total number of cells per surface unit of pineal section, decreased from birth to the end of the first month. This decrease was also steeper from birth to the 7th postnatal day than at any other period of the study. These results support the idea that a strong expansion of the cellular population of the rat pineal gland precedes morphological and functional maturation and opens the way to further exploration of the relationship between functional and proliferative responses of the pineal gland.
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    Characterization of GFAP expression and cell proliferation in the rat median eminence following hypophysectomy
    (F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2001) Vázquez, R.; Blanco, E.; Sánchez, F.; Juanes, J. A.; Rubio, M.; Santos, M.; Vázquez, G.; Hernández, E.; Riesco, J. M.; Carretero, J.
    To analyze whether the reorganization of the rat medi an emin ence aft er hypophysectomy might be related to changes in gli al fibrill ary ac idi c protein (GFAP)- and cellular prolife ration, th e distribution of cells immunoreactive for GFAP and the prolife ration rate of such cells were analyzed at 20, 40 and 60 days posthypoph ysec tomy. Fo r this stud y, four rostra -ca ud al regions of the medi an eminence we re diffe renti ated: the retroc hi as mati c, preinfundibul a r, infundi bul ar and postinfundibul ar regions. In each of these regions, three layers were studied: the ependymal, the internal and the ex tern al. At 20 and 40 days aft er hypoph ysec tomy, significant increases in ce llular proliferation affecting all three laye rs studi ed in th e pre infundi bul a r a nd infundibul ar regio ns we re fo und . At the same time po ints, in c reases in GFAP ex pr essio n we re a lso obse rved . Howeve r, a ft e r 60 days, GFA P a nd proliferative ce llular nuclear antigen (PCNA) expression dec reased. Although va ri ati ons of PCNA and GFA P levels were ev id ent, no coloca lisa ti on of PCNA and GFAP was fo und in the cells of the medi an eminence in untreated or hy pophysectomized rats when sections we re analyzed by double immunohistochemica l staining. Our results suggest that reorgani zation of med ian eminence in vo lves alt e rati o ns (o r modul ati o n) o f GFA Pimmunoreacti ve ce lls together with a proliferation of cells that are not GFAP-immunoreactive. This study also demonstrates that this reorgani zation is completed within the fi rst two months afte r hypophysectomy.