Browsing by Subject "Calretinin"

Now showing 1 - 4 of 4
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    Development of neurons and fibers containing calcium binding proteins in the pallial amygdala of mouse, with special emphasis on those of the basolateral amygdalar complex
    (Wiley, 2005-08-08) Legaz Pérez, Isabel; Olmos, Luis; Real, M. Ángeles; Guirado, Salvador; Dávila, José Carlos; Medina, Loreta; Ciencias Sociosanitarias
    We studied the development of neurons and fibers containing calbindin, calretinin, and parvalbumin in the mouse pallial amygdala, with special emphasis on those of the basolateral amygdalar complex. Numerous calbindin-immunoreactive (CB+) cells were observed in the incipient basolateral amygdalar complex and cortical amygdalar area from E13.5. At E16.5, CB+ cells became more abundant in the lateral and basolateral nuclei than in the basomedial nucleus, showing a pattern very similar to that of γ-aminobutyric acid (GABA)ergic neurons. Many CB+ cells observed in the pallial amygdala appeared to originate in the anterior entopeduncular area/ganglionic eminences of the subpallium. The density of CB+ cells gradually increased in the pallial amygdala until the first postnatal week and appeared to decrease later, coinciding with the postnatal appearance of parvalbumin cells and raising the possibility of a partial phenotypic shift. Calretinin (CR) immunoreactivity could be observed in a few cells and fibers in the pallial amygdala at E14.5, and by E16.5 it became a good marker of the different nuclei of the basolateral amygdalar complex. Numerous CB+ and CR+ varicosities, part of which have an intrinsic origin, were observed in the basolateral amygdalar complex from E16.5, and some surrounded unstained perikarya and/or processes before birth, indicating an early formation of inhibitory networks. Each calcium binding protein showed a distinct spatiotemporal expression pattern of development in the mouse pallial amygdala. Any alteration in the development of neurons and fibers containing calcium binding proteins of the pallial amygdala may result in important disorders of emotional and social behavior.
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    Immunocytochemical localization of the calcium-binding proteins calbindin D28K, calretinin and parvalbumin in bat visual cortex
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Kim, Hang-Gu; Gu, Ya-Nan; Lee, Kyoung-Pil; Lee, Ji-Gun; Kim, Chan-Wook; Lee, Ji-Won; Jeong, Tae-Hee; Jeong, Young-Wun; Jeon, Chang-Jin
    It is a common misconception that bats are blind, and various studies have suggested that bats have visual abilities. The purpose of this study was to investigate the cytoarchitecture of calbindin D28K (CB)-, calretinin (CR)-, and parvalbumin (PV)-immunoreactive (IR) neurons in the bat visual cortex using immunocytochemistry. The highest density of CB- and PV-IR neurons was located in layer IV of the visual cortex. The majority of CB- and PV-IR neurons were characterized by a stellate or round/oval shape. CR-IR neurons were predominantly located in layers II/III, and the cells were principally round/oval in shape. Two-color immunofluorescence revealed that 65.96%, 24.24%, and 77.00% of the CB-, CR-, and PV-IR neurons, respectively, contained gamma-aminobutyric acid (GABA). We observed calcium-binding protein (CBP)- IR neurons in specific layers of the bat visual cortex and in specific cell types. Many of the CBP-IR neurons were GABAergic interneurons. These data provide useful clues to aid in understanding the functional aspects of the bat visual system.
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    Ovarian Leydig cells (OLC): A histomorphological and immunohistochemical study
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Carrasco Juan, J.L.; Álvarez Argüelles Cabrera, H.; Martín Corriente, M.C.; González Gómez, M.; Valladares Parrilla, F.; Gutiérrez García, R.; Díaz Flores, L.
    Testicular Leydig cells (LC) regulate the proper development of male individuals, both during fetal life (fetal LC) and puberty (adult LC). In the ovaries of adult women, there are cells that are very similar to Leydig cells, the ovarian hilus cells (OHC), which also produce testosterone. The origin of these cells, in both sexes, remains unknown and is still a matter of debate. We have studied the location, characteristics and relationships of the OHC in 90 patients. The indications for oophorectomy were: metrorrhagia (n=9), prolapse (n=8), endometrial hyperplasia (n=14), cancer (endometrial, myometrial, or cervical) (n=35), uterine leiomyomata (n=14), and various ovarian tumors (cysts and benign tumors, borderline and malignant) (n=10). In addition to the hilus, occasionally the nodules, nests and clusters of OHC were located in the mesovarium, the mesosalpinx, and in the medullar and cortical regions of the ovaries. The morphological (including crystalloids of Reinke) and immunohistochemical (positivity for calretinin and alpha-inhibin) findings were similar to those described for testicular LC. Therefore, OHC can be considered ovarian Leydig cells (OLC). LC are usually found in small numbers in the ovaries, but if one looks for them intentionally, one always finds them. Close relationships were observed between the OLC with nerves and vessels. Moreover, an intraneural location of the OLC was demonstrated in all cases, and these intraneural cells showed similar characteristics to extraneural OLC, suggesting that they derive from endoneural cells which are present in the vegetative nerves of the ovaries.
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    Thrombomodulin, calretinin and c-kit (CD117) expression in cardiac myxoma
    (F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2001) Acebo, E.; Val Bernal, J. F.; Gómez Román, J. J.
    The immunohistochemica l profil e of cardiac myxoma has been debated. The tumor is thought to be de ri ve d fr om multipo te nti a l undiffe re ntiated mesenchymal cells. A consistent marker fo r this tumor has not bee n fo und . In this arti cle an immunohistoc hemi ca l stu dy of 23 ca rdi ac myxo mas was accomplished. This stud y comprised th e immunoreactivit y of the tumors fo r thrombomodulin, calretinin and and c-kit (COIl 7). To the best of our knowledge, th ro mb omodulin and c-kit have no t bee n tested in cardi ac myxoma. Ca lretinin expression has been recentl y demonstrated in cardiac myxoma, although this finding has not been yet validated. Surface lining ce lls, tumor vascular endothelium, cells around the vascular slits and stromal ce lls embedd ed in the myxoid matri x we re assessed independently. All tumors showed reactivity fo r thromb omodulin in th e surface ce lls and in th e end oth elium of neopl asti c vesse ls. 82.6% of ca rdi ac myxomas ex pressed th rombomodulin in the stromal cells and 69.6 % o f the tum o rs we re reac ti ve in th e pe ri vascul ar cells. 73.9% of cardiac myxomas expressed ca lretinin in th e stromal cells and in the peri vascul ar ce lls. All myxo mas we re nega ti ve fo r c- kit. Thrombomodulin and ca lretinin may be impo rtant diagnosti c aids for ca rdiac myxoma. Ca rdiac myxoma ce lls do no t exp ress e mbr yo ni c/fe tal e ndo th e li a l antigens.