Browsing by Subject "C-reactive protein"

Now showing 1 - 9 of 9
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    Analytical validation of commercially available methods for acute phase proteins quantification in pigs
    (Elsevier, 2006-12-01) Tecles Vicente, Fernando; Fuentes, P.; Martínez Subiela, Silvia; Parra Muñoz, María Dolores; Muñoz, A.; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    The aim of this study was to validate commercially available methods for porcine haptoglobin (Hp), C-reactive protein (CRP), serumamyloid A (SAA) and major acute phase protein (Pig-MAP) determinations. Intra and inter assay coefficients of variation (CVs) were lower than 20% in all cases with exception of inter assay CVs for CRP and Pig-MAP assays with samples of low acute phase proteins concentration, and for SAA assay at any acute phase proteins concentration. All methods showed good linearity and detection limits were low enough to detect APPs levels in healthy animals. Hp and SAA were very affected by haemolysis. Lipaemia influenced mainly on SAA determination. Over 15-fold increase was observed in CRP and SAA concentrations after artificially induced inflammation by a single subcutaneous dose of turpentine, whereas Hp and Pig-MAP increased less than 5-fold.
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    C-reactive protein and atherosclerosis. Is there a causal link?
    (Murcia : F. Hernández, 2003) Manolov, D.E.; Koenig, W.; Hombach, V.; Torzewski, J.
    C-reactive protein (CRP) is a powerful cardiovascular risk marker. Evidence suggests that this may be due to its direct proatherogenic properties. Because of different biological functions of CRP in different species, an appropriate animal model for the study of its role in atherogenesis is difficult to set up. Binding to low density lipoprotein (LDL), activation of the complement system and interaction with monocyte/macrophages are rigorously defined pathogenic properties of CRP which might contribute to an active role of the molecule in human atherogenesis. Furthermore, direct effects on arterial wall cells, i.e. endothelial cells and smooth muscle cells, have been reported. The molecular basis of CRP interaction with these cells, however, remains unclear. Should CRP indeed be actively involved in human atherogenesis, the molecule may become a target for therapy. Pharmaceutical companies develop CRP-inhibitors.
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    C-Reactive protein levels in community acquired pneumonia
    (European Respiratory Society (ERS), 2002-10-24) García-Vázquez, Elisa; Martínez, J.A.; Mensa, J.; Sánchez, F.; Marcos, M.A.; Roux, A. de; Torres, A.; Medicina
    The diagnostic value of C-reactive protein (CRP) admission serum levels as an indicator of the aetiology of community acquired pneumonia (CAP) was evaluated. A cohort of 1,222 patients with CAP was assessed. CRP levels were analysed in 258 patients with a single aetiological diagnosis. The mean CRP values in patients with pyogenic, atypical, viral and Legionella pneumophila pneumonia were: 16 mg?dL-1 , 13 mg?dL-1 , 14 mg?dL -1 and 25 mg?dL-1 , respectively. CRP levels were not significantly different among patients outcome research team (PORT) groups (19 mg?dL -1 in groups I–II, 16 mg?dL-1 in group III and 16 mg?dL -1 in groups IV–V. A cut-off point of 25 mg?dL-1 had a sensibility, specificity, positive predictive value and negative predictive value of 0.6, 0.83, 0.3, and 0.94, respectively. After controlling for age and PORT score, the odds of having a CRP level w25 mg?dL-1 was 6.9 times higher in patients with L. pneumophila pneumonia than in those with non-L. pneumophila pneumonia. Patients with Legionella pneumophila pneumonia had higher C-reactive protein levels than those with pneumonia of any other aetiology, independently of severity of infection. Being a cheap and readily available test, C-reactive protein may be a useful adjunctive procedure in the diagnosis of community acquired pneumonia.
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    C-reactive protein measurement in canine saliva
    (SAGE Publications, 2005-03) Parra Muñoz, María Dolores; Tecles, Fernando; Martínez Subiela, Silvia; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    An established time-resolved immunofluorometric assay designed for measurement of C-reactive protein (CRP) in canine blood was evaluated and validated for use in canine saliva. C-reactive protein was measured in saliva specimens from 5 healthy dogs before and after the injection of casein and in 37 dogs with different disease conditions. The analytical and functional limits of detection were 0.000053 mg/ml and 0.0091 mg/ml, respectively, and intra- and interassay coefficients of variation ranged between 6.7–9.9% and 8.5–16.5%, respectively. A recovery experiment showed no significant disagreement between detected values and expected ones, and saliva CRP concentration was measured in a linear and proportional manner. A positive correlation was found between CRP levels obtained in saliva and serum samples in the experimental (R2 5 0.76) and clinical studies (R2 5 0.70). The assay was able to detect significant differences between salivary CRP levels in healthy dogs and dogs with inflammatory processes. These results suggest that saliva can be used for CRP measurement in dogs. The use of saliva presents the advantage of an easier and less stressful sampling method for the animals, which might be performed outside of hospital environments.
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    C-reactive protein measurements in meat juice of pigs
    (Elsevier, 2008-04-15) Gutiérrez, A.M.; Martínez Subiela, Silvia; Montes, A.; Parra Muñoz, María Dolores; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    A time-resolved immunofluorometric assay was evaluated for measurement of C-reactive protein in meat juice from diaphragmatic muscle collected from slaughtered pigs. Analytical and clinical validation of the method was performed by using meat juice samples, obtained by freezing and thawing muscle pieces. The intra- and inter-assay coefficients of variation ranged from 2.2–5.8% to 7.9–14.3%, respectively. The limit of detection was 0.00038 mg/ml. The method measured the CRP concentrations in a linear manner with a good accuracy (r = 0.99). CRP concentrations in serum were highly correlated with those in diaphragmatic meat juice (r = 0.90; p < 0.001). CRP concentrations were significantly higher in clinically affected pigs compared to non-diseased pigs. The assay described here provides a sensitive method for measuring CRP concentrations in meat juice, which can represent a suitable alternative to serum or blood samples and simplifies the process of sampling collection at slaughter.
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    Evaluation of C-reactive-like protein in Mytilus galloprovincialis
    (Elsevier, 2019-07-05) Franco-Martínez, Lorena; Tvarijonaviciute, Asta; Mateo, Sandra V.; Cerón, José J.; Romero, Diego; Oliveira, Miguel; Teles, Mariana; Martínez-Subiela, Silvia; Ciencias Sociosanitarias
    C-reactive protein (CRP) is an acute phase protein of the innate immune system with an important role as biomarker in many biological processes in mammals. However, there is a lack of information regarding CRP in molluscs and there is no data about the presence of CRP-like protein in mussels. In the present work, CPR-like protein was identified and purified from the digestive gland of the Mediterranean mussel Mytilus galloprovincialis In addition, it was demonstrated that CRP-like protein can be measured accurately and precisely by a high- throughput automated method. Finally, higher levels of CRP-like protein in mussels exposed to hypoxia and nanoplastics were detected when compared to controls. The results obtained indicate that CRP-like protein could be a suitable novel biomarker of inflammatory response in mussels for biomonitoring programs being economic, easy and rapid to determine.
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    Saliva as a non-invasive tool for assessment of metabolic andinflammatory biomarkers in children
    (Elsevier, 2020-08) Tvarijonaviciute, Asta; Martínez Lozano, Nuria; Ríos, Rafael; Marcilla de Teruel, María C.; Garaulet, Marta; Cerón, José J.; Medicina y Cirugía Animal
    Background&aims:Epidemiological studies in school-age children are challenging, particularly thosethat aim to analyse metabolic markers on blood samples obtainedviainvasive and stressful procedures.The objective of this paper is to evaluate the use of saliva, as a non-invasive tool in epidemiologicalstudies performed in school-age children, to capture metabolic changes associated with body mass index(BMI), dietary characteristics and physical activity in both boys and girls.Methods:This is an observational study in which healthy children of ages between 8 and 12 years(n¼129, 60 girls and 69 boys) from three schools in a Mediterranean area of Spain were included. Apanel of biomarkers was measured in serum and saliva and correlated with BMI, dietary characteristicsand physical activity.Results:Significant positive correlation between serum and salivary levels were detected for CRP(r¼0.770) in all included children, and boys (r¼0.805) and girls (r¼0.775) separately (P<0.001, in allcases) and for insulin in girls (r¼0.442;P<0.05). Among all studied salivary biomarkers, insulin wassignificantly correlated with the three factors studied: positively with BMI and negatively with dietarycharacteristics (intake and composition) and physical activity (P<0.05). Obesity and diet compositionwere both positively associated to pro-inflammatory biomarkers, CRP and IL1b; while diet compositionshared with physical activity levels the correlation with IL6 (positive with energy, fat, carbohydrate andsaturated fatty acid intake, and negative with cholesterol intake and average physical activity in boys),NGF and glucose (in both cases correlations were negative with diet composition and physical activityvariables) (P<0.05, in all cases). Sex differences were detected in serum glucose and TNFa.Conclusions:Biomarkers in saliva are able to capture differences in BMI, dietary characteristics andphysical activity levels in school-age children. Saliva may potentially constitute a useful non-invasive andstress-free tool to evaluate metabolic markers of inflammation and/or metabolism related to BMI andlifestyle in a sex-dependent manner.
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    Serum acute phase response induced by different vaccination protocols against circovirus type 2 and Mycoplasma hyopneumoniae in piglets
    (Elsevier, 2017-03-11) Hernandez-Caravaca, Iván; Figueras Gourgues, Sebastian; Rodríguez, Víctor; Díaz Estrada, Edgar; Cerón Madrigal, José Joaquín; Escribano Tortosa, Damián; Producción Animal
    The purpose of this study was to compare the acute phase reaction (APR) induced by different vaccination pro- tocols used against Porcine Circovirus (PCV) type-2 and Mycoplasma hyopneumoniae (M.hyo), studying two acute phase proteins (APPs) and changes in rectal temperature (RT). In addition, the possible influence of the time of vaccination and breed were analysed. In the first experiment, 40 commercial crossbred piglets were vaccinated, on the day of weaning, with FLEXcombo® (group A, n = 20) or Porcilis PCV® and Stellamune® One (group B, n = 20). The second experiment was performed on two farms, on which 40 commercial crossbred piglets or 40 Iberian piglets were vaccinated, 7 days post-weaning. On each farm one group (A, n = 20) was vaccinated with FLEXcombo® and another group (B, n = 20) with Porcilis® PCV-M.hyo. Blood samples were taken before, 24 h and 48 h after vaccination, and RT were recorded before and 8 h after vaccination. Significantly higher in- creases in group B in RT (P b 0.01) and APPs concentrations (P b 0.01) were recorded at several sampling times after vaccination compared with group A. The vaccines that produced greater increases in RT also produced higher APPs increases but no influence of the day of vaccination or of the breed were found. Therefore, serum APPs concentrations differed according to the vaccine used, which may be useful, along with RT, for choosing the vaccine or protocol that produces APR of lower magnitude.
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    Utilidad de las proteínas de fase aguda en el líquido cefalorraquídeo canino para el diagnóstico de la me-ningoencefalomielitis de origen desconocido
    (Universidad de Murcia. Servicio de publicaciones, 2024) Verdú Serrano, María Elena; Muñoz Prieto, Alberto; Mateo Pampliega, Isidro; García Martínez, Juan Diego
    El sistema inmunitario facilita la defensa de los seres vivos, que se desencadena a través de la respuesta de fase aguda, generándose las proteínas de fase aguda. Estas proteínas, medidas en suero, resultan de utilidad. El uso de biomarcadores para el diagnóstico precoz de enfermedades inflamatorias del sistema nervioso central (SNC), está en auge en medicina humana. En el caso de las meningoencefalomielitis de origen desconocido (MOD), no hay estudios con una información relevante que sea capaz de solventar el gran inconveniente de no poder obtener un diagnóstico definitivo, que no sea de manera postmortem. La medición de proteínas de fase aguda, como la proteína C reactiva (CRP) y la haptoglobina en el líquido cefalorraquídeo (LCR) podría ayudar a solventar este problema. El objetivo de esta investigación es valorar si estas proteínas se pueden detectar en el LCR, mediante un analizador bioquímico automatizado y estudiar la existencia de alteraciones DOI: 10.6018/analesvet.562071 en animales con MOD. Para ello, los animales incluidos en el estudio se dividieron en un grupo control, com-puesto por perros sin MOD y un grupo problema, subdividido en perros con MOD y perros con MOD tratados con corticoides. Para la medición de estos parámetros se utilizó el analizador Olympus AU600®. Para realizar el estudio comparativo entre grupos se aplicó la prueba no paramétrica de Kruskal-Wallis, seguido del test de Dunn para comparaciones múltiples. La CRP no mostró variaciones estadísticamente significativas, mientras que la haptoglobina no se pudo detectar en el LCR, por lo que se debería valorar su medición con técnicas más sensibles que permitan su cuantificación.