Browsing by Subject "Células madre, telómeros y telomerasa"

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    Cáncer y el envejecimiento desde el final de los cromosomas.
    (2007-10-29) Urbina, Luis
    Células madre, telómeros y telomerasa: una visión del cáncer y el envejecimiento desde el final de los cromosomas. Conferencia a cargo de Ignacio Flores, del Centro Nacional de Investigaciones Oncológicas (CNIO, Madrid). Organiza: Facultad Biología UMU. Aula 1.6 de la Facultad de Biología. Campus de Espinardo. (26-10-07).
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    Evaluación de la longitud telomérica relativa leucocitaria en bovinos nacidos por reproducción asistida
    (Universidad de Murcia. Facultad de Veterinaria, 2025-09-22) Andrés Zúñiga, Paula; Heras García, Sonia; Coy Fuster, María Pilar; Sin departamento asociado; Facultades de la UMU::Facultad de Veterinaria
    Located at the ends of chromosomes, telomeres are a fundamental tool for protecting the integrity of genetic material. Their sequence is regenerated after each cell division by the enzyme telomerase and other molecular mechanisms, preventing the loss of genetic information. However, not all cells can restore their telomere length. This results in their progressive shortening, which, upon reaching a critical length, induces cell death mechanisms in cells. In the cattle industry, assisted reproductive technologies offer many advantages in terms of animal production and genetic characteristics. Nevertheless, it is unknown whether these technologies could induce and/or accelerate telomere shortening in individuals derived from them. The aim of this study was to analyse the possible influence of assisted reproductive technologies on relative telomere length in bovine leukocytes throughout the animals' lives. The relationship between telomere length and the sex and survival of the individuals was also evaluated. Samples were obtained from 19 cows and bulls from a previous study. Relative leukocyte telomere length was determined by quantitative PCR after DNA extraction. Two primers were designed, one to amplify telomeric fragments and the other for the single-copy reference gene GTF2I. A significant influence of assisted reproductive technologies on relative leukocyte telomere length was observed. Specifically, the transfer of embryos produced in vitro, with culture medium supplemented with reproductive fluids, was the technique associated with longer telomere length. In contrast, artificial insemination was associated with the shortest telomere length values. However, no significant associations were observed between telomere length and the sex or survival of the animals. These results suggest that relative leukocyte telomere length appears to be influenced by the type of assisted reproductive technology. Yet, further research would be desirable to complement and validate the differences observed and to investigate their possible relationship with sex and longevity.