Histology and histopathology Vol. 5, nº 3 (1990)

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Browsing Histology and histopathology Vol. 5, nº 3 (1990) by Subject "Histochemistry"

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    Histological features and histochemistry of the mucous glands in ventral skin of the frog (Rana fuscigula)
    (Murcia : F. Hernández, 1990) Els, Willem J.; Henneberg, Renata
    The glycoconjugate components of secretory granules were analyzed in cells of mucous glands in ventral skin from Rana fuscigula. The analysis was done with standard histochemical methods on semithin glycol methacrylate-embedded tissues. The staining patterns in semithin sections were comparable to those using paraffin-embedded tissue while the cytological detail was better preserved. The mucous glands contained at least two different types of secretory cells lining the lower two-thirds of the mature gland: a principal cell type filled with dense staining secretory granules and a solitary type containing paler staining, globular secretory granules. The principal type of cell contained variable amounts of acid glycoconjugates; predominantly carboxylated but also variably carboxylated and weakly sulfated glycoproteins. Other secretory cells contained mainly neutral glycoproteins. The results indicated that the mucus is a heterogeneous substance and that one cell type may produce different secretory products. We suggested that the variability in histochemical staining might be related to the sequence of biosynthesis of the secretory granule.
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    Variations in fibre composition of the gastrocnemius muscle in rats subjected to speed training
    (Murcia : F. Hernández, 1990) Morales-López, J. L.; Agüera, E.; Miró, F.; Diz, A.
    Thirty-six adult Wistar rats were divided into three groups. One group was used as a control, and the other two underwent different training programmes in which greater relevance was attached to the intensity of exercise than to its duration. Samples of the red and mixed portions of m. gastrocnemius (caputlateralis) were stained with m-ATPase to determine the percentage of type 1, IIA and IIB fibres, and with NADH-TR in order to quantify variations in the percentage of low staining intensity (FG) fibres. The most notable results obtained were: a) the ratio of type 1 type 11 fibres remained unchanged; b) the proportion of IIA fibres increased, while that of IIB fibres decreased correspondingly; c) FG fibres, which were virtually absent from the red portion, recorded a clear decrease which was more marked, and occurred more rapidly, than in IIB fibres. These differences were al1 statistically significant in the mixed portion of the muscle. Adaptative changes in fibre composition in the red portion were less marked.