Histology and histopathology Vol. 4, nº 3 (1989)

Permanent URI for this collection

https://hdl.handle.net/10201/17693

Browse

Now showing 1 - 13 of 13

Open Access
Ulmtrastructuroef the chronically vagotomised atrial myocardiu m in t he mon key (Macaca fascicularis)
(Murcia : F. Hernández, 1989) Wong, W. C.; Yick, T. Y.; Ling, E. A.
The ultrastructure of the atrial myocardium in the monkey (Macaca fascicularis) was studied after bilateral cervical vagotomy and survival times of 100,175 and 367 days. Changes were observed in the nucleus and the cytoplasm of the myocyte. Sequestered within the nuclei of the affected myocytes were cytoplasmic organelles and inclusions. In the late stages, there was a tendency towards condensation and margination of the heterochromatin. Changes in the cytoplasm included increased glycogen, mitochondrial degeneration and myofibrillar disorganisation and degeneration. There was increased collagen and mononuclear cell infiltration in the extracellular space in the later stages. This study has shown that the long term structural integrity of the atrial myocyte depends on an intact vaga1 innervation. The survival of the monkey after chronic bilateral vagotomy suggests that this nonhuman primate is a suitable model for functional studies of the parasympathetically denervated heart.
Open Access
IBL-like T cell lymphoma expressing monoclonal gammopathy (macroglobulinemia) in the serum
(Murcia : F. Hernández, 1989) Kasajima, Takeshi; Masuda, Akihiro; Matsuda, Mikio; Imai, Yutaka; Tobinai, Kensei
A case of IBL-like T cell lymphoma with serum monoclonal gammopathy was reported. A 58- year-old woman, who had suffered from heart failure. was admitted because of asthma attack, fever and lyrnphadenopathy. Leucopenia with a small amount of atypical lymphocytes was detected. Serum analysis showed monoclonal elevation of IgM-K (M-protein) and hv~erviscositv. Urinarv Bence-Jones urotein was diiected. Lymph &de biopsy rAealed the disappearance of normal structure and proliferation of T cells with pale cells which characterized IBL-like T cell lymphoma. Immunocytochemistry revealed the pale cells to bear T cell markers (MT-1, CD 5, CD 8 or CD 4) and IgM-positive cell distribution. Tonsilar biopsy showed the infiltration of atypical lymphoids and pale cells. Bone marrow biopsy showed moderate lymphoplasmacytoid proliferation with lymph follicles. Clinical data and serum analysis suggested macroglobulinemia. Additional lymph node biopsy was performed and revealed IBL-like T cell lymphoma. IBLlike T cell lymphoma is characterized by polyclonal hypergammaglobulinemia. The present case probably occurred initially as IBL-like T cell lymphoma and lymphoplasmacytoid cell proliferation might have followed due to an excess of CD 4' cells
Open Access
Liver-cell adenoma in an epileptic man on barbiturates
(Murcia : F. Hernández, 1989) Jaime Vázquez, J. Jaime; Marigil, Miguel A.
The case of a 19-year-old epileptic man with a solitary hepatic adenoma is described. The tumor was 9 x 8.5 x 6.5 cm in size and microscopically consisted of cells similar in appearance to non-neoplastic hepatocytes, arranged in cords with slit-like sinusoids interposed. Bile ducts and portal tracts were conspicuously absent. Our patient was on antiepileptic drugs, among them phenobarbital which experimentally produces liver cell tumors in mice and rats.
Open Access
Surface morphology and ultrastructure of normal and cyclic hematopoietic canine bone marrow in long-term liquid cultures
(Murcia : F. Hernández, 1989) Al-Lebban, Z.S.; Lange, R.D.; Jones, J.B.; Lothrop, Jr.
Long-term liquid cultures of normal and cyclic hematopoietic (CH) dog bone marrow produce committed granulocyte-macrophage progenitor cells (CFU-GM) and differentiated granulocytes for severa1 weeks. Analysis of in situ fixed cultures or of cells harvested from the culture supernatants revealed that the cells had ultrastructure and surface morphology characteristic of immature and mature rnyeloid cells. The surface rnorphologies of adherent cells from both normal and CH dogs were similar. The characteristic abnormalities previously reported in neutrophils obtained from CH dogs were not observed in neutrophils obtained from long-term marrow cultures of CH dogs. These results indicate that the cellular abnormalities in the neutrophils of CH dogs may be secondary manifestations of the disease and are not inherent to the pathogenesis of the hematopoietic cells.
Open Access
Ultrastructure of atrial and ventricular myocytes of newborn rats: evidence for the existence of specific atrial granule-like organelles in the ventricle
(Murcia : F. Hernández, 1989) Venance, Shannon L.; Pang, Stephen C.
The present study examined the ultrastructure of atrial and ventricular myocytes from the heart of newborn rats. It was found that, despite former reports stating that ventricular myocytes in adults do not contain cytoplasmic granules, specific atrial granule-like organelles are present in the ventricles of rats at birth. The presence of these granules together with the relatively underdeveloped contractile apparatus and extensive Golgi complex suggests that the ventricular, like the atrial, myocytes may have an endocrine function before or at birth. Further study is required to determine whether these ventricular cytoplasmic granules contain the same atrial natriuretic peptide species known to be present in the atrial specific granules.
Open Access
Histochemistry and morphometry of Werdnig-Hoffmann disease
(Murcia : F. Hernández, 1989) Pérula, E. Artacho; Villalobos, R. Roldán; Lernos, R. Vaamonde
We report a case of the Werdnig-Hoffmann disease in a 4-month-old male infant. The morphological study revealed perimysial fibrosis. variability in the size of muscle fibers, absence of target fibers, few central nuclei and normality in vessels, nerves and neuromuscular junctions. The morphometrical examination showed the existence of normal-sized and atrophic fibers in both fibrillar types. as well as in hypertrophic type 1 fibers. The percentage of fibrillar types and the data obtained from the form factor are normal. Random distribution of type 1 and 11 muscle fibers were observed.
Open Access
Regional adaptation of Müller cells in the chick retina. A Golgi and electron microscopical study
(Murcia : F. Hernández, 1989) Prada, F. A.; Espinar, A.; Chmielewski, C. E.; Dorado, M. E.; Genís-Gálvez, J. M.
We report the morphological differences of Müller cells in relation to their topography, using the Golgi method. Müller cells in the central retina are long and slender, with numerous inner prolongations. In the peripheral retina, the morphology of the Müller cells adapts to the reduced thickness of the retina1 layers. In this zone, they are short and have thick inner prolongations which end in a large foot in the internal limiting membrane. In the optic disc margin, Müller cells have a particular morphology characterized by thick, arched prolongations that in general form a glial network between the retina and optic nerve. The ultrastructure of these cells is also described. The results are discussed with respect to the nature of Müller cells.
Open Access
Retinal pigment epithelial fine structure in the bobtail goanna (Tiliqua rugosa)
(Murcia : F. Hernández, 1989) Braekevelt, Charlie R.
The retinal pigment'epithelium (RPE), the choriocapillaris and Bruch's membrane (complexus basalis) have been studied by light and electron microscopy in the bobtail goanna (Tiliqua rugosa) an Australian diurna1 lizard. The RPE consists of a single layer of cuboidal cells which display very deep and tortuous basal (choroidal) infoldings as well as numerous apical (vitreal) processes which interdigitate with the photoreceptor cells. The lateral cell borders are relatively smooth and joined by basally located tight junctions. Internally smooth endoplasmic reticulum is abundant while rough endoplasmic reticulum is not. The RPE cell nucleus is large and vesicular and basally located in the light-adapted state. Polysomes, mitochondria and myeloid bodies are present and widely distributed. Melanosomes are plentiful in the apical region of the epithelial cells in light-adaptation. Bruch's membrane is pentalaminate with the basal lamina of the choriocapillaris being exceptionally thick. The choriocapillaris is a single layer of large-caliber capillaries with thin but only moderately fenestrated endothelium. Numerous dense granules are always present within these endothelial cells.
Open Access
Retinal pigment epithelial fine structure in the bobtail goanna (Tiliqua rugosa)
(Murcia : F. Hernández, 1989) Braekevelt, Charlie R.
The retinal pigment'epithelium (RPE), the choriocapillaris and Bruch's membrane (complexus basalis) have been studied by light and electron microscopy in the bobtail goanna (Tiliqua rugosa) an Australian diurna1 lizard. The RPE consists of a single layer of cuboidal cells which display very deep and tortuous basal (choroidal) infoldings as well as numerous apical (vitreal) processes which interdigitate with the photoreceptor cells. The lateral cell borders are relatively smooth and joined by basally located tight junctions. Internally smooth endoplasmic reticulum is abundant while rough endoplasmic reticulum is not. The RPE cell nucleus is large and vesicular and basally located in the light-adapted state. Polysomes, mitochondria and myeloid bodies are present and widely distributed. Melanosomes are plentiful in the apical region of the epithelial cells in light-adaptation. Bruch's membrane is pentalaminate with the basal lamina of the choriocapillaris being exceptionally thick. The choriocapillaris is a single layer of large-caliber capillaries with thin but only moderately fenestrated endothelium. Numerous dense granules are always present within these endothelial cells.
Open Access
A morphological study on the reproductive organs as a possible cause of developmental abnormalities in diabetic NOD mice
(Murcia : F. Hernández, 1989) Tatewaki, Reiko; Otani, Hiroki; Tanaka, Osamu; Kitada, Jin-ichi
The reproductive organs in non-obese diabetic (NOD) mice were histopathologically studied, in order to elucidate the relationships between developmental abnormalities, such as diminkhed rates of implantation and viable embryos, and structural changes in the reproductive organs. NOD mice with (NOD-DM) and without (NOD-N) diabetes mellitus and ICR mice were compared. The severity of histopathological changes in the pancreas and in the liver were used as parameters which indicated the severity of diabetes itself and of the secondary metabolic disorder. NOD-DM mice exhibited uterine weight loss, accumulation of lipids in luminal and glandular epithelium, atrophies of the endometrium and myometrium and a decrease in the number of muscle cell layers. They also showed a high concentration of lipid droplets in ovarian granulosa cells, atretic follicles and atrophy and lack of lipids in ovarian stroma cells. The severity of these structural changes in the reproductive organs corresponded to those of the changes in the pancreas and the liver. The structural alterations in the ovary suggested disorder in oocyte maturation. The structural changes in the uterus appeared to be related to the decrease in the ratios of implantation and of viable embryos at post-implantation stage. The present studies suggest that the impaired structural environment together with the metabolic environment caused the abnormal development seen, for example. in the oocyte maturation, and at the implantation and post-implantation stage of diabetic mice. It also caused alterations in their hormonal environment
Open Access
The value of conjunctival biopsy in childhood cystinosis
(Murcia : F. Hernández, 1989) Cruz-Sánchez, F. F.; Cervós-Navarro, J.; Rodríguez-Prados, S.; Lennert, T.
Cystinosis is frequently presented with cystine storage in the cornea and cnnjiinctiva. and the diagnosis can be established by slit-lamp esamination. It can also be confirmed by elcctron microscopy of a conjunctival biopsy . The present paper reports on n 16-nionth-old boy with Fanconi's syndronie. in whoni the slit-lanip examination did not show crystal deposits of cystinc in the conjunctiva. The ~iltrastructurals tudj,o f the conjunctival biopsy demonstruted polygonal crystals within douhle mcmbrane-liniited organelles located in fibrobliists. Similar crystals were subsequently found in a hidney biopsy. We therefore think that conjunctival biopsy is a valuable diagnostic tool prior to pcrforming renal biopsy, even in cases with negativc findings hy ophthalmologic examination.
Open Access
Fine structure of the conus papillaris in the bobtail goanna (Tiliqua rugosa)
(1989) Braekevelt, Charlie R.
The structure of the conus papillaris in an Australian lizard. the bobtail goanna (Tiliqua rugosa) was investigated by lipht and electron microscopy. In this strongly diurrial species, the conus papillaris consists of a heavily vascularized and pigmented, finger-like structure about 1 mm ir1 diameter and 3-3 mm in length. It is situated over the optic nerve head and projects into the vitreous chamber. Within the conus are numerous capillaries and larger blood vessels. melanocytes and occasional mast cells. Many of the capillaries display prominent luminal and abluminal microfolds. Other capillaries show no microfolds while still others display an intermediate number of microfolds. The larger blood vessels are usually indistinguishable as to being either arterioles or venules. The endothelial cells of al1 blood vessels show a population of cytoplasmic granules. The melanocytes are large pleomorphic cells usually rich in microfilaments. Unmyelinated nerve processes are plentiful within the conus and the Schwann cells ericlosing these nerve fibres are occasionally seen to be pigmented. The rnorphology of the conus papillaris indicates a heavy involvernent in the transport of materials. It is considered to be homologous to the pecten oculi of the avian eye: to the falciform process of the teleost eye; to the supraretinal vessels of amphibians and to the intraretinal vessels of the mammalian eye.
Open Access
Regional adaptation of Müller cells in the chick retina. A Golgi and electron microscopical study
(Murcia : F. Hernández, 1989) Prada, F. A.; Espinar, A.; Chmielewski, C. E.; Dorado, M. E.; Genís-Gálvez, J. M.
We report the morphological differences of Müller cells in relation to their topography, using the Golgi method. Müller cells in the central retina are long and slender, with numerous inner prolongations. In the peripheral retina, the morphology of the Müller cells adapts to the reduced thickness of the retina1 layers. In this zone, they are short and have thick inner prolongations which end in a large foot in the internal limiting membrane. In the optic disc margin, Müller cells have a particular morphology characterized by thick, arched prolongations that in general form a glial network between the retina and optic nerve. The ultrastructure of these cells is also described. The results are discussed with respect to the nature of Müller cells.

Browse

Browsing Histology and histopathology Vol. 4, nº 3 (1989) by Issue Date

Results Per Page

Sort Options