DigitalUM :: Browsing by browse.metadata.contributordepartment "Biología Celular e Histología"

Browsing by browse.metadata.contributordepartment "Biología Celular e Histología"

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Open Access
17alpha-ethynylestradiol prevents the natural male-to-female sex change in gilthead seabream (Sparus aurata L.)
(Springer Nature, 2020-11) García Hernández, M.P.; Cabas, I.; Rodenas, M.C.; Arizcun, M.; Chaves-Pozo, E.; Power, D.M.; García Ayala, Alfonsa; Biología Celular e Histología
Open Access
17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream
(Elsevier, 2017) Gómez González, Nuria Esther; Cabas, Isabel; Rodenas, María del Carmen; Arizcun, Marta; Mulero Méndez, Victoriano Francisco; García Ayala, Alfonsa; Biología Celular e Histología
17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor. Nevertheless, these previous studies have mainly focused on the head kidney leukocytes and no information exists on peritoneal leukocytes, including mast cells. In the present work, juvenile gilthead seabream fish were fed a pellet diet supplemented with EE2 for 76 days and intraperitoneally injected with hemocyanin plus imject alum adjuvant at the end of EE2 treatment and 92 days later, and the peritoneal immune response was analyzed. EE2 supplementation induced vtg expression but returned to basal levels by 3 months post-treatment. Interestingly, gilthead seabream peritoneal leukocytes express the genes encoding for the nuclear estrogen receptor α and the G protein-coupled estrogen receptor 1 and the dietary intake of EE2 induced these expression. Moreover, EE2 induced an inflammatory response in the peritoneal cavity in unvaccinated fish, which was largely maintained for several months after the cessation of the treatment. However, the impact of EE2 in vaccinated fish was rather minor and transient. Taken together, the study provides fresh information about endocrine immune disruption, focusing on peritoneal leukocytes.
Open Access
A low nuclear-to-cytoplasmic ratio of VDR expression is an independent prognostic marker in breast cancer
(Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Schubert Charlotte; Vilsmaier Theresa; Batz Falk; Cavaillès Vincent; Sixou Sophie; Kolben Thomas; Meister Sarah; Buschmann Christina; Hagemann Friederike; Biología Celular e Histología
The aim of this retrospective study was to analyze the prognostic value of cytoplasmic versus nuclear expression of the vitamin D receptor (VDR) in breast cancer (BC) tissue samples and to relate the results to clinicopathological parameters. VDR expression was assessed in 319 primary breast cancer patients using the Remmele and Stegner immunoreactive scoring (IRS) system. Follow-up data were obtained from the Munich Cancer Registry. The correlation with overall survival (OS) and disease-free survival (DFS) was calculated using univariate and multivariate analyses. Correlation analysis revealed a correlation between nuclear VDR expression and improved outcomes for both OS (p=0.004) and DFS (p=0.001). Conversely, cytoplasmic VDR expression was significantly associated with a shorter OS (p=0.003) and DFS (p<0.001). Additionally, both cytoplasmic and nuclear VDR expression were found to be independent markers of DFS (p<0.001; p=0.021) when examined alongside clinicopathological parameters. Moreover, nuclear VDR expression was positively associated with lower lymph node invasion (pN; p=0.01). For triple-negative patients, cytoplasmic VDR expression was found to have a significant inverse correlation with DFS (p<0.001). Lastly, the ratio of VDR nuclear/cytoplasmic was identified as an auxiliary independent marker of DFS and OS. These findings strongly indicate that the subcellular localization of VDR is crucial in determining BC prognosis. The expression of nuclear VDR appears to have a protective effect, while cytoplasmic VDR is associated with a more aggressive disease course. The data may help identify subgroups of patients with high-risk BC, possibly leading to specific options for targeted tumor therapy
Open Access
A new approach to study inflammation in fish: Serum proteinogram analysis in gilthead seabream (Sparus aurata) injected with λ-carrageenan
Campos Sánchez, Jose Carlos; Esteban Abad, María Ángeles; Guardiola, Francisco A.; Biología Celular e Histología
Open Access
A simple format feed to test the acceptability of ingredients for common octopus (Octopus vulgaris Cuvier, 1797)
(Wiley, 2015-03-10) Sánchez Morillo-Velarde, Mª Piedad; Cerezo Valverde, Jesús; García García, Benjamín; Biología Celular e Histología
Open Access
Adaptive changes in the visual cortex after photoreceptor degeneration in retinitis pigmentosa
(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Martinez Galan, Juan R.; Caminos, Elena; Biología Celular e Histología
Retinitis pigmentosa (RP) is a group of hereditary disorders that cause progressive retinal degeneration, affecting the rods and, subsequently, the cones, which results in progressive vision loss. RP is genetically heterogeneous and is inherited in an autosomal dominant, autosomal recessive, X-linked, or sporadic non-Mendelian manner. The recent advance-ments in repairing damaged retinas highlight the necessity of understanding the impact of photoreceptor degeneration on the visual cortex. This is because functional vision may not be adequately restored if this region is significantly impaired prior to treatment. In the present review, we have analyzed the rodent models of RP that have been most frequently used and the physiological and morphological changes occurring in both humans and rodents with this disorder. Following visually evoked stimulation, the processing of visual information in the primary visual cortex (V1) of individuals with RP is altered due to modifications in the transduction of the signal originating in the degenerated retina. Moreover, alterations in the intrinsic electro-physiological properties of cortical neurons and neural circuits have also been documented. Finally, several neurochemical and/or morphological changes are observed in synaptic structures associated with pyramidal neurons and in select inhibitory interneurons. Nevertheless, despite the physiological and morphologi-cal changes that have been described, the impact of RP on the visual cortex does not inevitably result in irreversible damage, as the alterations do not appear to be particularly severe. Brain plasticity is more restricted in adults; however, remodeling of the visual cortex in mice and humans is possible, which encourages further work on therapies capable of partially restoring the lost visual function.
Open Access
Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining
(Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Yu Chao; Liu Xiao; Zhao Peiyuan; Sun Zhidong; Song Yurong; Cao Yuan; Cheng Ming; Biología Celular e Histología
In this study, two chromogenic systems, horseradish peroxidase (HRP)-3,3’-diaminobenzidine (DAB) and HRP-3,3',5,5'-tetramethylbenzidine (TMB), were used to perform single-color and double-color immunohistochemical staining (sIHC and dIHC, respectively) on multiple antigens in four distinct tissue types. The chromogenic results of the HRP-TMB system exhibited a vibrant blue-green color, and the tissue localization and signal intensity were consistent with those of the HRP-DAB system. In addition, it demonstrated clear differentiation from the hematoxylin-stained nucleus, endogenous melanin, and brown chromogenic results of HRP-DAB. TMB staining in tissues that contain high endogenous pigment levels eliminates the need for melanin bleaching, thereby facilitating direct observation and potentially improving the detection speed and interpretation. TMB can also be used in combination with DAB for dIHC, thus allowing detection of the two markers on a single slide. However, the TMB staining results are not stable over the long term and require image storage using slide scanners, thereby limiting its application. Additionally, in dIHC, overlapping signals of the first marker may obscure the second marker, potentially leading to bias or false negatives. Therefore, careful consideration is required when designing dIHC detection systems. Based on the above, we propose that TMB is a valuable supplement to DAB for immunohistochemical staining and deserves further promotion and utilization. However, additional research is needed to improve the composition of TMB chromogenic reagents, prolong the longevity of staining results, and overcome current limitations
Open Access
Aluminum is a powerful adjuvant in teleost fish despite failing to induce interleukin-1β release
(2018-08) Angosto, Diego; López-Muñoz, Azucena; García-Alcázar, Alicia; Meseguer, José; Sepulcre Cortés, María Pilar; Biología Celular e Histología
Open Access
An infrared investigation in relation with chitin and chitosan characterization
(Elsevier, 2001-01-12) Brugnerotto, J.; Lizardi, J.; Goycoolea Valencia, Francisco Martín; Argüelles Monal, Waldo; Desbrières, J.; Rinaudo, Marguerite; Biología Celular e Histología
The use of infrared spectroscopy for characterization of the composition of chitin and chitosan covering the entire range of degree of acetylation (DA) and a wide variety of raw materials is examined further. The ratio of absorbance bands selected was calibrated using 1H liquid and 13C CP-MAS solid-state NMR as absolute techniques. IR spectra of the structural units of these polymers validated the choice of baselines and characteristic bands. The bands at 1650 and 1320 cm-1 were chosen to measure the DA. As internal reference, the intensities at 3450 and 1420 cm-1 were evaluated. The absorption band ratios involving the reference at 3450 cm-1 had poorer fit. The absorption ratio A1320/A1420 shows superior agreement between the absolute and estimated DA-values (DA% = 31.92A1320/A1420 - 12.20; r = 0.990)
Open Access
Analysis of ZP1 gene reveals differences in zona pellucida composition in carnivores
(2017) Moros-Nicolás, C.; Leza, A.; Chevret, P.; Guillén-Martínez, A.; González-Brusi, L.; López-Béjar, M.; Ballesta, J.; Avilés, M.; Izquierdo-Rico, M.J.; Biología Celular e Histología
Open Access
Aptamer-Target-gold nanoparticle conjugates for the quantification of fumonisin B1.
(MDPI, 2021-01-08) Vicente A Mirón-Mérida; Yadira González-Espinosa; Mar Collado-González; Yun Yun Gong; Yuan Gong; Francisco M Goycoolea; Biología Celular e Histología; Facultades de la UMU
Fumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive and selective; however, they also convey disadvantages such as long assay times, expensive equipment and instrumentation, complex procedures, sample pretreatment and unfeasibility for on-site analysis. Therefore, there is a need for quick, simple and affordable quantification methods. On that note, aptamers (ssDNA) are a good alternative for designing specific and sensitive biosensing techniques. In this work, the assessment of the performance of two aptamers (40 and 96 nt) on the colorimetric quantification of FB1 was determined by conducting an aptamer–target incubation step, followed by the addition of gold nanoparticles (AuNPs) and NaCl. Although MgCl2 and Tris-HCl were, respectively, essential for aptamer 96 and 40 nt, the latter was not specific for FB1. Alternatively, the formation of Aptamer (96 nt)–FB1–AuNP conjugates in MgCl2 exhibited stabilization to NaClinduced aggregation at increasing FB1 concentrations. The application of asymmetric flow field-flow fractionation (AF4) allowed their size separation and characterization by a ultidetection system (UV-VIS, MALS and DLS online), with a reduction in the limit of detection from 0.002 g/mL to 56 fg/mL.
Open Access
Assessing acute pancreatitis: A novel method combining live cell imaging with tissue damage evaluation
(Universidad de Murcia, Histología e Histopatología, 2025) Kovačič Polona; Skelin Klemen Maša; Paradiž Leitgeb Eva; Venglovecz Viktória; Kiss Lóránd; Mihalekné Fűr Gabriella; Stožer Andraž; Dolenšek Jurij; Biología Celular e Histología
Acute pancreatitis (AP) is a sudden inflammation of the exocrine part of the pancreas, resulting in self-digestion and destruction of exocrine tissue. The intricate relationship between exocrine and endocrine functions is pivotal, as damage to acinar cells can affect endocrine cell function and vice versa. However, our understanding of these interactions remains limited. An effective strategy for investigating pancreatic cells involves the utilization of live in-situ acute mouse pancreas tissue slice preparations, combined with noninvasive fluorescent calcium labeling of endocrine or exocrine cells, and subsequent analysis using confocal laser scanning microscopy. Nevertheless, this approach encounters inherent conflicts with conventional methodologies employed to histologically assess the severity of tissue damage due to AP in the model. Traditional methods involve fixing and staining tissue samples with hematoxylin and eosin, thereby precluding live-cell imaging. In this study, our objective was to introduce an innovative method utilizing a commercial fluorescence Live/Dead assay that enables calcium imaging and tissue damage assessment in the same sample. This approach was validated against the classical histological grading of AP severity, and we found a good correlation between the classical histological grading method and the in-situ approach employing the Live/Dead assay. The primary advantage of our novel approach lies in its capacity to enable timely and efficient live-cell imaging together with damage assessment in the same tissue, thereby enabling the study of functional consequences of structural damage at the cellular level and reducing the number of animals required for experimentation.
Open Access
Assessing chemical toxicity of ionic liquids on Vibrio ﬁscheri: Correlation with structure and composition
(Pergamon – Elsevier Science Ltd, 2016-04-11) Hidalgo, Juana M; García Montalbán, Mercedes; Salinas Hidalgo, María Dolores; Collado-González, Mar; Díaz Baños, F. Guillermo; Víllora Cano, Gloria; Biología Celular e Histología
One of the most important properties of the ionic liquids (ILs) is their non-volatility. However, they are wide soluble in water. For this reason, they can be released to aquatic ecosystems and to contribute to water pollution. Nevertheless, toxicological data related to ionic liquids is scarce in literature because of the great number of possible ionic liquids synthesized. The present work reports the toxicity of twenty-nine imidazolium-, pyridinium- and ammonium-based ionic liquids towards the luminescent bacteria Vibrio fischeri. Some of the effects analyzed on the toxicity have been the type of anion, the length of the alkyl chain of the cation, the cation core and the presence of a functionalized side chain in the cation. These results have showed that the main influence on the toxicity of the ILs is the alkyl chain length. A Quantitative Structure-Activity Relationships (QSAR) method has been used to validate our results obtaining a very good agreement.
Open Access
Associative phenomena in galactomannan-deacetylated xanthan systems
(Elsevier, 2001-09-30) Goycoolea Valencia, Francisco Martín; Milas, Michel; Rinaudo, Marguerite; Biología Celular e Histología
The interaction between mesquite seed galactomannan (MSG; D-mannose to D-galactose ratio (M/G) ~1.1) and deacetylated xanthan (DX) in 5 mM NaCl leading to synergistic gel formation at 25 °C was investigated and compared with the far more studied system made of xanthan and locust bean gum (LBG; M/G ~3.5). Rheology and differential scanning calorimetry were used to measure temperatures of gel formation and transition enthalpy as a function of polymer composition, while circular dichroism was used to probe the conformation of DX in the LBG– DX system. MSG and DX associate at 25 °C with a well defined stoichiometry of 0.6:1.0 (w/w) at low ionic strength favouring the disordered coil state of DX. When LBG was used in place of MSG in water or 5 mM NaCl, two types of mechanisms of interpolymeric association are envisaged.
Open Access
Astragaloside IV suppression of chronic atrophic gastritis by upregulating PAR-1 in vitro and in vivo
(Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Duan Bensong; Bao Zhewei; Yang Jingya; Wang Zhenzhen; Li Aoxiang; Yang Jin; Lv Mengke; Zhang Haibin; Biología Celular e Histología
Background. Astragaloside IV (AS-IV) has demonstrated a protective effect against gastrointestinal tract injury induced by various factors. However, its potential mechanism against chronic atrophic gastritis (CAG) remains unknown. Purpose. The objective of the present study was to investigate the impact of AS-IV on CAG and elucidate its molecular mechanism. Methods. The mRNA and protein levels of protease-activated receptor-1 (PAR-1) and related proteins were assessed using reverse transcription-polymerase chain reaction and western blot analyses, respectively. In addition, the levels of inflammatory factors were measured via enzyme-linked immunosorbent assay in GES-1 cells following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The CAG model was established in rats induced with MNNG and concurrently treated with AS-IV for 10 weeks. Subsequently, serum samples were collected to assess the expression levels of proteins reflecting inflammatory markers. The gastric tissue sections were used for hematoxylin and eosin staining, immunohistochemical analysis, and the assessment of p-NF-κB p65 and PAR-1 signaling. Results. In-vitro experiments demonstrated that the mRNA levels of PAR-1 were upregulated following treatment with AS-IV and MNNG. Conversely, inhibition of PAR-1 expression reversed the therapeutic effects of AS-IV on MNNG-treated GES-1 cells, leading to increased expression of cyclooxygenase-2 and p-NF-κB p65. In addition, PAR-1 inhibition notably reversed MNNG-induced inflammatory factors, including IL increase. In-vivo experimental validation further confirmed that the upregulation of PAR-1 expression following treatment with AS-IV exerted a protective effect on the gastric mucosa of CAG rats. Conclusion. In conclusion, the findings of the present study suggested that AS-IV exhibited therapeutic efficacy against CAG induced by MNNG; its mechanism may be closely associated with the thrombin/PAR-1 signaling pathway. The present study provides a theoretical foundation for further exploration of the pharmacological effects of AS-IV on the treatment of human CAG
Open Access
Beneficial roles of feed additives as immunostimulants in aquaculture: A review
(2018) Dawood, Mahmoud A.O.; Koshio, Shunsuke; Esteban Abad, María Ángeles; Biología Celular e Histología
The use of antibiotics in the aquafeeds to mitigate infectious diseases or to boost growth performance is commonly practiced. Recently, the prophylactic use of antibiotics and chemotherapies have been criticized which eventually led to their ban in aquaculture by law on many countries. In view of the current restrictions on antibiotic use, there is a critical need to evaluate other possible alternatives. One potential substitute for antibiotics in aquafeeds is the use of functional feed additives. The present review is a comprehensive and an updated compilation of the available works on growth promoters and novel feed supplements, their examples, modes of action and useful applications for improving aquafeeds. It highlights probiotics, prebiotics, synbiotics, immunostimulants, organic acids, nucleotides and medicinal herbs. Apart from boosting aquafeeds and safeguarding general health of aquatic animals, some of these substances have been found to possess beneficial immunostimulant and anti-stress relieving properties. Furthermore, the use of these natural substances increases the consumer confidence of farmed fish. Due care has been taken to cover the ongoing trends and recent advances with a perspective vision and their holistic usages and beneficial applications in aquatic animals’ systems. The current study also shed light regarding the management and production aspects of aquatic animals which will enlighten farmers and producers for better economic growth.
Open Access
Biocompatibility of new pulp-capping materials NeoMTA Plus, MTA Repair HP and Biodentine on human dental pulp stem cells
(Elsevier Science, 2017-11-01) Tomás Catalá, Christopher Joseph; Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Forner, Leopoldo; Llena, Carmen; Lozano, Adrián; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Biología Celular e Histología
Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, Neo-MTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp-capping materials was determined by energy-dispersive X-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energydispersive X-ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp-capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time-dependent.
Open Access
Bioinformatic and gene expression analysis of the somatostatin/cortistatin gene family in the gilthead seabream (Sparus aurata).
Campos Sánchez, Jose Carlos; Serna‑Duque, Jhon A.; Guardiola, Francisco A.; Cuesta, Alberto; Esteban Abad, María Ángeles; Biología Celular e Histología
Open Access
Biomarker-based assessment of somatostatin-6 immunomodulation in gilthead Seabream: From serum proteins to skin mucus enzymes
(0022-10-20) Jose Carlos Campos Sánchez; Cristóbal Espinosa-Ruiz; Claudia Marín-Parra; Francisco A. Guardiola; María Ángeles Esteban; Biología Celular e Histología
This study evaluated the immunomodulatory role of somatostatin-6 (SST6) in gilthead seabream (Sparus aurata) using a validated inflammation model induced by λ-carrageenan and a comprehensive panel of serum and skin mucus biomarkers. SST6 was administered at 1 nM and 2 nM, and its effects were assessed in these matrices. The λ-carrageenan model triggered a local inflammatory response with reduced peroxidase, esterase, and protease activities in skin mucus, consistent with the resolution phase of innate immunity. SST6 at 1 nM enhanced serum complement activity without affecting acute-phase proteins, suggesting reinforcement of basal immune surveillance in the host. When combined with λ-carrageenan, SST6 promoted an increase in serum immunoglobulin levels and a selective shift in proteinogram fractions (α2-globulin), while further decreasing the oxidative and proteolytic activities in skin mucus. Correlation analysis revealed the coordinated regulation of key mucosal enzymes, reinforcing the functional integration of local immune responses. These results indicate that SST6 facilitates an early transition from innate to adaptive immunity while preserving the integrity of the mucosal barrier. Furthermore, this study provides the first evidence of complement activation by SST6 in fish and highlights the value of integrating biomarker-based tools to monitor immunophysiological responses and test bioactive compounds in aquaculture research.
Open Access
Biomechanical and histological analyses of a multilayer stent in a swine model of suprarenal aortic aneurysm
(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Tobita, Allana Maryel; Strazzi, Anna Paula Weinhardt Baptista; Portugal, Maria Fernanda Cassino; Wolosker, Nelson; Aun, Ricardo; Monteiro, Frederico de Lima Jacy; Vieira da Silva, Luiz; Sincos, Igor Rafael; Biología Celular e Histología
Objectives. To analyze and compare, in an animal model, the treatment of thoracoabdominal aneurysms with multilayer stents and its hemodynamic effects through the biomechanical and histological analysis of the aortic wall in contact with the stent. Methods. Large White pigs were randomized into two groups: Stent (n=6) and Control (n=5, non-stent). All animals were subjected to the creation of a suprarenal aneurysm with a bovine pericardial patch. In the Stent group, a multilayer stent was implanted immediately after aneurysm formation. After four weeks, all animals were subjected to angiographic assessment and intravascular ultrasound, and the stent was explanted before euthanasia for histological and biomechanical analyses. Results. At histological analysis, the groups did not differ significantly in maximum thickness of the intima (p=0.526), media (p=0.129), or adventitia (p=0.662). Thrombus formation was observed in 100% of the animals on the intima and media layers of the stented aorta vs. none in the Control group (p=0.048). At biomechanical analysis, no statistical differences were observed in aortic wall elasticity (p=0.158), strength (p=0.360), or thickness (p=0.323). Conclusion. We identified thrombosis of the aneurysmal sac through the presence of thrombi on the intima of the aorta in 100% of the animals in the Stent group; as for the biomechanical analysis, this study showed no statistical differences in vessel wall thickness, strength, and elasticity between

Browsing by browse.metadata.contributordepartment "Biología Celular e Histología"

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