Histology and histopathology Vol.24, nº4 (2009)

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Browsing Histology and histopathology Vol.24, nº4 (2009) by Author "Ferreira Oton-Leite1, Angélica"

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    Density and migration of mast cells in lip squamous cell carcinoma and actinic cheilitis
    (Murcia : F. Hernández, 2009) Lago Costa, Nádia; Ferreira Oton-Leite1, Angélica; Peixoto Cheim-Júnior, Adonai; Gonçalves Alencar, Rita de Cássia; Jabur Bitta, Glória Oton; Aparecida Silva, Tarcília; Carvalho Batista, Aline
    Mast cells (MCs) display a diversity of roles that may contribute to the stromal microenvironment alterations during tumor progression. The aim of this study was to investigate MC populations expressing tryptase and c-kit in lip squamous cell carcinoma (lip SCC) (n=37), actinic cheilitis (AC) (n=15) and normal lip mucosa (control) (n=6), as well as their relationship with microscopic parameters (collagen degeneration, elastin changes, angiogenesis and proliferative index). Tryptase, c-kit, CD31 and Ki-67 expressions were analyzed by means of immunohistochemistry and collagen and elastic fibers were visualized with Picrosirus and Verhoeff’s stain, respectively. The numbers of tryptase+ MC were significantly higher in lip SCC when compared with control (P=0.01), while a similar density of these cells was observed in AC and lip SCC (P=0.09). The density of c-kit+ MC was similar in all groups examined (P=0.65). MC migration (ckit+/Tryptase+ relationship) was 69% in lip SCC, 60% in AC and 100% in control. The number of CD31+ blood vessels was significantly higher in the lip SCC when compared with control and AC (P<0.01). The increase of MCs and angiogenesis in lip SCC may reflect an important modification in the tumor microenvironment during squamous photocarcinogenesis.