Publication: Short term regulation of hepatocyte glutathione content by hepatic sinusoidal cells in co-culture
Date
2007
Authors
Catalá-Rodríguez, M. ; Pagani, R. ; Portolés, M.T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
antioxidant mechanisms may constitute the
primary mechanisms of a number of pathologies. The
liver plays a central role in this balance: parenchymal
hepatic cells contain and export especially high levels of
the antioxidant glutathione and activated Kupffer cells
release inflammation mediators and reactive oxygen
species. There is growing evidence of a paracrine
regulation of hepatic function by means of a fluent
intercellular communication which must still be fully
elucidated, especially in basal conditions. In vivo models
provide often too complex results but, in vitro, tissue
interactions are left aside; therefore it is important to
find new experimental models to address cell
communication studies. Here we propose the
complementary use of three models to study liver
glutathione system regulation in basal conditions: pure
parenchymal cells primary cultures, addition of
sinusoidal cell conditioned media to parenchymal cells
and co-culture of sinusoidal cells using porous
membranes. We have also developed a high specifity
immunofluorescent method for the complete
characterization of sinusoidal cell populations by flow
cytometry and confocal microscopy. Our results show
that Kupffer cells possess higher levels of reactive
oxygen species than sinusoidal endothelial cells even in
basal conditions. We also report that the glutathione
content of hepatic parenchymal cells in basal conditions and suggest the existence of a paracrine circuit in the
management of liver oxidative stress.
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