Publication: Early human trophoblast cell cultures.
A morphological and immunocytochemical study
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Date
1989
Authors
Logothetou-Rella, H. ; Kotoulas, I-G. ; Nesland, Jahn M. ; Kipiotis, D. ; Abazis, D.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/bookPart
Description
Abstract
Rapidly growing cytotrophoblasts were
isolated from early human chorionic villi and the
Papanicolaou method was used to characterize their
cytology and transformation into syncytiotrophoblasts.
Cytotrophoblasts fused and formed binucleated cells or
mononucleated intermediate cells. Syncytial cells were
formed by fusion of small cytotrophoblasts or intermediate
cells and cytotrophoblasts. Glycosaminoglycans were
produced in cytotrophoblasts and released extracellularly .
Here they were accumulated andlor diffused into a
continuous layer covering the cells. Glycosaminoglycans
in syncytial cells were contained in well defined
membranous sacs. Cytotrophoblasts only grown beyond
confluence differentiated into villi with a villus-like
histology.
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