Publication: Cytoprotection by pyruvate through an anti-oxidative mechanism in cultured rat calvarial osteoblasts
Loading...
Date
2006
Authors
Moriguchi, N. ; Hinoi, E. ; Tsuchihashi, Y. ; Fujimori, S. ; Iemata, M. ; Takarada, T. ; Yoneda, Y.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Although we have previously shown drastic
cell death by pyruvate deficiency in osteoblasts at the
proliferative stage, the exact mechanism remains unclear
so far. Cell survivability was significantly decreased in
rat calvarial osteoblasts cultured for 0 to 3 days in vitro
(DIV) following replacement of the eutrophic a-
modified minimum essential medium (a-MEM) with
Dulbecco’s modified eagle medium (DMEM) for
cultivation. The addition of pyruvate enriched in a-
MEM, but not in MEM, entirely prevented cell death
induced by the medium replacement throughout a
culture period from 0 to 3 DIV. Both cysteine and
reduced glutathione protected cell death in cells cultured
for 3 DIV without significantly affecting that in cells
cultured for 1 DIV, however, while none of lactate,
acetate and insulin significantly prevented the cell death
irrespective of the culture period up to 3 DIV. A marked
increase was detected in intracellular reactive oxygen
species (ROS) levels 4 h after the medium replacement.
In osteoblasts cultured in a-MEM for 3 DIV, but not in
those for 7 DIV, hydrogen peroxide (H2O2) markedly
decreased cell survivability when exposed for 2 to 24 h.
Furthermore, H2O2 was effective in significantly
decreasing cell survivability in osteoblasts cultured in
DMEM for 7 DIV. Pyruvate at 1 mM not only prevented
cell death by H2O2, but also suppressed the generation of
intracellular ROS in osteoblasts exposed to H2O2. These results suggest that pyruvate could be cytoprotective
through a mechanism associated with the anti-oxidative
property rather than an energy fuel in cultured rat
calvarial osteoblasts.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.