Publication: Matrix metalloproteinase 2 (MMP-2) and tissue transglutaminase (TG 2) are expressed in
periglandular fibrosis in horse mares with endometrosis
Authors
Walter, I. ; Handler, J. ; Miller, I. ; Aurich, C.
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Publisher
Murcia : F. Hernández
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Description
Abstract
Periglandular arrangement of myofibroblasts,
associated with the deposition of extracellular matrix
(ECM), is a cardinal feature of endometrosis in mares.
We hypothesized that a disturbance in the expression of
matrix degrading enzymes such as matrix
metalloproteinases (MMP’s) and matrix cross-linking
proteins might lead to an imbalance in deposition and
degradation of extracellular matrix components and
thereby accentuate degeneration. Therefore, distributions
of MMP-2, capable of collagen IV and laminin
degradation, and tissue transglutaminase (TG2), a crosslinker
of extracellular matrix proteins, were investigated
by means of immunohistochemistry on uterine biopsies
of healthy mares and animals with endometrosis. It was
illustrated that both proteins were present in fibrotic
regions of affected endometria, and that they were in
most cases colocalized. Periglandular MMP-2
expression was significantly associated with dilated and
fibrotic uterine glands. Furthermore, MMP-2 and TG 2
were demonstrated in the stratum compactum of healthy
and endometrotic endometria. Gelatin zymography
proved that active and inactive pro-form of MMP-2 were
present in all examined samples with significantly higher
amounts of total and active MMP-2 in affected
endometria. TG 2-activity, determined by an in situ
assay, was found in cases of severe periglandular fibrosis. We suggest that both enzymes play a major role
in changes that occur in ECM homeostasis in
endometrial fibrotic regions.
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