Publication: The effects of nitric oxide inhibition prior to
kainic acid treatment on neuro- and gliogenesis
in the rat dentate gyrus in vivo and in vitro
Authors
Cosgrave, A. Siobhan ; McKay, Jennifer S. ; Morris, Richard ; Quinn, John P. ; Thippeswamy, Thimmasettappa
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Treatment with the nitric oxide synthase
(NOS) inhibitor, L-NAME prior to the induction of
seizures with kainic acid (KA) [L-NAME+KA]
increases the expression of activity-dependent
neuroprotective protein (ADNP) in cells in the
subgranular zone (SGZ) of the rat dentate gyrus 3-days
after seizure induction (Cosgrave et al., 2009). Using the
incorporation of BrdU we found that this protocol [LNAME+KA]
stimulates neuro- and gliogenesis. By
comparison, L-NAME or KA alone produced smaller
effects. Doublecortin+ (BrdU negative) neuroblasts in
the SGZ also significantly increased with L-NAME+KA
treatment, suggesting that L-NAME+KA cause more
cells to differentiate into neurons.
L-NAME alone increased BrdU+ astrocytes in the
hilus implying that NO inhibits stem cell differentiation
into astrocytes and may also influence their migration.
Although NOS inhibition increased cell proliferation in
vivo and in vitro it disrupted cell clustering as revealed
by ADNP immunoreactivity. In vitro KA treatment
resulted in eccentric nuclei, reduced neurite extension
and branching in neurons and retracted processes of glia
cells, these changes were inhibited with prior treatment
of L-NAME suggesting that KA-induced NO production
affects cell morphology. Consequently, this data suggests
an important role for NO in regulating stem cell
proliferation and their fate in the SGZ.
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