Publication: Changes in MMPs and inflammatory cells in experimental gingivitis
Loading...
Date
2009
Authors
Lorencini, Márcio ; Silva, Juliete A.F. ; de la Hoz, Cristiane L.R. ; Carvalho Hernandes, F. ; Stach-Machado Dagmar, R.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
In periodontal disease, extensive
disorganization of the extracellular matrix promotes the
loss of adhesion between the teeth and periodontium. A
previous study suggested a reduction in the area
occupied by collagen in the gingiva, during the first
week of periodontal disease induction, however, the
remaining fibers were more compact and thicker.
Therefore, it was decided to investigate which of the
MMP-2, -9, -14 and RECK, an MMP inhibitor, were
involved in these modifications taking place in early
gingivitis induced by ligature. The results of gene
expression analysis indicated no changes for RECK.
MMP-14 showed a reduction at 7 days of inflammation,
and there was an immediate increase in MMP-2 gene
expression and enzymatic activity, apparently by the
stimulation of resident cells such as fibroblasts. A peak
of MMP-9 expression 5 days after ligature followed after
the peak of enzymatic activity found two days earlier.
This pattern was consistent with the kinetics of
macrophage and neutrophil recruitment. Immunohistochemistry suggested that MMP-9 was produced by
both resident and inflammatory cells. Based on this
evidence, it is suggested that extracellular matrix
remodeling is related to MMP-2 and -9 production and
activation. This allowed us to conclude that the host
inflammatory response represents a significant factor for
the advance of periodontal diseases.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.