Publication:
Absence of galectin-3 promotes neuroprotection in retinal ganglion cells after optic nerve injury

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Abreu-32-253-262-2017.pdf(3.84 MB)
Date
2017
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Authors
Abreu, Carla Andreia ; De Lima, Silmara Veline ; Mendonça, Henrique Rocha ; Oliveira Goulart, Camila de ; Blanco Martinez, Ana Maria
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-788
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info:eu-repo/semantics/article
Description
Abstract
A trauma to the mature central nervous system (CNS) often leads to persistent deficits, due to the inability of axons to regenerate after being injured. Increasing evidence suggests that pro-inflammatory and pro-apoptotic genes can present a major obstacle to promoting neuroprotection of retinal ganglion cells and consequently succeed in axonal regeneration. This study evaluated the effect of the absence of galectin-3 (Gal-3) on retinal ganglion cells (RGC) survival and axonal regeneration/degeneration after optic nerve crush injury. Two weeks after crush there was a 2.6 fold increase in the rate of cell survival in Gal-3-/- mice (1283±79.15) compared to WT animals (495.4±53.96). However, no regeneration was observed in the Gal-3-/- mice two weeks after lesion. Furthermore, axonal degeneration presented a particular pattern on those mice; Electron Microscopy (EM) analysis showed incomplete axon degeneration while the WT mice presented an advanced stage of degeneration. This suggests that the removal of the nerve fibers in the Gal 3-/- mice could be deficient and this would cause a delay in the process of Wallerian degeneration once there is a decrease in the number of macrophages/microglia in the nerve. This study demonstrates how the absence of Gal-3 can affect RGC survival and optic nerve regeneration/degeneration after lesion. Our results suggest that the absence of Gal-3 plays an important role in the survival of RGC and thus can be a potential target for therapeutic intervention in RGC neuroprotection.
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Retinal ganglion cells , Optic nerve , Galectin-3 , Wallerian Degeneration
Citation
Histology and Histopathology, Vol.32, nº3, (2017)
URI
http://hdl.handle.net/10201/117026
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Histology and histopathology Vol.32, nº3 (2017)
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