Publication: a1-acid glycoprotein (AGP): a possible carrier of
sialyl lewis X (slewis X) antigen in colorectal carcinoma
Authors
Croce, M.V. ; Sálice, V.C. ; Lacunza, E. ; Segal-Eiras, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objectives: 1- to detect a1-acid glycoprotein
(AGP) and sialyl Lewis x (sLex) in colorectal malignant,
benign and normal samples; 2- to isolate AGP from
colorectal cancer and 3- to study its immunoreactivity
with an anti-sLex monoclonal antibody (MAb).
Materials and methods: tissue and serum samples from
88 patients with colorectal cancer, 22 adenomas and 23
normal were included. Expression of AGP and sLex was
studied by immunohistochemistry (IHC); isolation
approach: AGP was precipitated with ammonium
sulphate and immunoprecipitated with anti-AGP MAb.
The immune complex formed was isolated by protein ASepharose
CL-4B affinity chromatography and further
eluted; fractions were analysed by SDS-PAGE and
Western-blot. Statistical analysis was performed by
means of Principal Component Analysis. Results: by
Western blot employing anti-AGP MAb and sLex
MAbs, isolated fractions from malignant samples
showed a band at about 45kD. IHC revealed that AGP
was expressed in 70% of colorectal carcinoma samples,
50% of benign and 35% of normals. SLex was detected
in 31% of malignant samples, 41% of benign and in one
normal sample. In malignant samples, AGP reaction
comprised the whole specimen with a strong and
homogeneous staining while normal and benign samples
showed a restricted reaction. In cancer, sLex expression
consisted in an intense reactivity in membrane, cellular
debris and some cytoplasmic foci while normal and
benign samples were occasionally stained. A statistically
significant positive correlation was found between AGP
and sLex expression. Serum AGP levels were measured
by radial immunodiffusion and statistical comparative
analysis with tissue expression did not show a
correlation between both parameters. Conclusion: AGP
may constitute a carrier of sLex in colorectal cancer.
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