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Latexin expression correlated with mineralization of articular cartilage during progression of post-traumatic osteoarthritis in a rat model

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dc.contributor.authorMartínez Calleja, América
dc.contributor.authorCruz, Raymundo
dc.contributor.authorMiranda Sánchez, Magdalena
dc.contributor.authorFragoso Soriano, Rogelio
dc.contributor.authorVega López, Marco A.
dc.contributor.authorKour, Juan B.
dc.date.accessioned2022-11-09T17:21:27Z
dc.date.available2022-11-09T17:21:27Z
dc.date.issued2020
dc.description.abstractAs latexin has been linked with chondrocyte hypertrophic differentiation it is possible that this protein may also be involved in the mineralization of cartilage in OA. Therefore, we correlated latexin expression with the mineralization marker, alkaline phosphatase and determined the mineral deposition in the articular cartilage by analyzing the Ca/P ratio and the collagen fibrils pattern, during the progression of post-traumatic OA in a rat model. OA was induced by medial meniscectomy and post-surgery exercise for 5, 10, 20 and 45 days. Protein expression in articular cartilage was evaluated by immunofluorescence, histochemistry and Western blot. Minerals and structure of collagen fibrils in the superficial zone of cartilage were analyzed by energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) respectively. Protein expression analysis showed time-dependent up- regulation of latexin during OA progression. In the cartilage, latexin expression correlated with the expression and activity of alkaline phosphatase. EDX of the superficial zone of cartilage showed a Ca/P ratio closer to theoretical values for basic calcium phosphate minerals. The presence of minerals was also analyzed indirectly with AFM, as the collagen fibril pattern was less evident in the mineralized tissue. Latexin is expressed in articular cartilage from the early stages of post-traumatic OA; however, minerals were detected after latexin expression was up-regulated, indicating that its activity precedes and remains during the pathological mineralization of cartilage. Thus, our results contribute to the identification of molecules involved in the mineralization of articular chondrocytes.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 35, nº 3 (2020)
dc.identifier.doihttps://doi.org/10.14670/HH-18-151
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/125388
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectOsteoarthritises
dc.subjectChondrocyte hypertrophyes
dc.subjectMineralizationes
dc.subjectArticular cartilagees
dc.subjectLatexines
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleLatexin expression correlated with mineralization of articular cartilage during progression of post-traumatic osteoarthritis in a rat modeles
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.35, nº3 (2020)