Publication: Ultrastructural changes of the human enteric nervous system and interstitial cells of Cajal in diverticular disease.
Authors
Alaburda, Paulius ; Lukosiene, Jaune I. ; Pauza, Audrys G. ; Kyguoliene, Kristina Rysevaite ; Kupcinskas, Juozas ; Saladzinskas, Zilvinas ; Pauziene, Neringa
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-18-136
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. In spite of numerous advances
in understanding diverticular disease, its pathogenesis
remains one of the main problems to be solved. We
aimed to investigate the ultrastructural changes of the
enteric nervous system in unaffected individuals, in
asymptomatic patients with diverticulosis and in patients
with diverticular disease.
Methods. Transmission electron microscopy was
used to analyse samples of the myenteric, outer
submucosal and inner submucosal plexuses from
patients without diverticula (n=9), asymptomatic patients
with diverticulosis (n=7) and in patients with
complicated diverticular disease (n=9). We described the
structure of ganglia, interstitial cells of Cajal and enteric
nerves, as well as their relationship with each other. The
distribution and size of nerve processes were analysed
quantitatively.
Results. In complicated diverticular disease, neurons
exhibited larger lipofuscin-like inclusions, their
membranous organelles had larger cisterns and the
nucleus showed deeper indentations. Nerve remodeling
occurred in every plexus, characterised by an increased
percentage of swollen and fine neurites. Interstitial cells
of Cajal had looser contacts with the surrounding cells
and showed cytoplasmic depletion and proliferation of
the rough endoplasmic reticulum. In asymptomatic
patients with diverticulosis, alterations of enteric nerves
and ICC were less pronounced.
Conclusions. In conclusion, the present findings
suggest that most ultrastructural changes of the enteric
nervous system occur in complicated diverticular
disease. The changes are compatible with damage to the
enteric nervous system and reactive remodeling of
enteric ganglia, nerves and interstitial cells of Cajal.
Disrupted architecture of enteric plexuses might explain
clinical and pathophysiological changes associated with
diverticular disease
publication.page.subject
Citation
Histology and Histopathology Vol. 35, nĀŗ 2 (2020)
item.page.embargo
Ir a EstadĆsticas
Este Ćtem estĆ” sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/