Publication: A study of myonecrosis induced by the venom of the scorpion tityus serrulatus
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Date
1987
Authors
Luque, E. ; Martín, J.D. ; Peña, J. ; Roldan, R. ; Vaarnonde, R.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The pathogenesis of skeletal muscle necrosis
produced by Tityus Serrulatus venom was studied by
means of light microscopy and electron microscopy.
Wistar rats were inoculated subcutaneously, at some
distance from the muscles under study, with a sublethal
dose of scorpion venom. Samples were taken of the
tibialis anterior muscles of both rear legs, 2, 7 and 24
hours postinoculation. Light microscopy analysis after 2
hours revealed certain changes identified as «delta
lesionw, and also the presence of hyperconcentrated
muscle cells. Electron microscopy confirmed these
lesions and also enabled us to identify a degree of
discontinuity in the plasma membrane with a persistence
of the basa1 membrane. Hyperconcentrated fibers could
still be observed 7 hours postinoculation. Histochemical
analysis revealed high levels of calcium within the fibers.
24 hours after inoculation with the venom, numerous
phagocytic cells were found in the degenerated fibers.
Muscle cells were also found to have undergone
alterations indicative of an ischemic process. The most
characteristic finding 7 days postinoculation was the
appearance of regenerative fibers. After thirty days the
muscles regained their normal appearance. It is suggested
that Tityus Serrulatus venom induces myonecrosis by
means of a twofold action: direct action, which gives rise
in the first place to a rupture of the plasma membrane,
permitting a massive entry of calcium this being a key
factor in the process of cell lession and an assumed
indirect action due to ischemia.
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