Publication: Use of laser scanning cytometry
to study tumor microenvironment
Authors
Mocellin, S. ; Wang, E. ; Panelli, M. ; Rossi, C.R. ; Marincola, F.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The study of phenomena occurring in the
tumor microenvironment is a challenging task because
of technical difficulties, particularly when dealing with
hypocellular specimens. Laser scanning cytometry
(LSC) is a new laboratory technology that has been
recently introduced to overcome the limitations of other
traditional technologies. By combining the properties
and the advantages of flow cytometry (FC) and
immunohistochemistry (IHC), LSC allows the
investigator to obtain objective information on DNA
content, protein expression and cellular localization is
combination with morphological features. It has been
already shown that LSC results are reliable compared to
more traditional technologies, and its implementation in
the clinical routine is under way. Its use in oncology,
which is rapidly expanding, spans from apoptosis
analysis to DNA content quantitation and tumor cell
phenotyping.
Here we describe the technology underlying this
novel fluorescence-based device, review its use in
oncology by dissecting the phenomena occurring in the
tumor microenvironment and propose its application for
the immunological follow-up of malignant lesions
undergoing immunotherapeutic manipulation.
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