Publication: Role of skeletal muscle in mandible development
Authors
Rot, Irena ; Mardesic-Brakus, Snjezana ; Costain, Willard J. ; Saraga-Babic, Mirna ; Kablar, Boris
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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Description
Abstract
As a continuation of the previous study on
palate development (Rot and Kablar, 2013), here we
explore the relationship between the secondary cartilage
mandibular condyles (parts of the temporomandibular
joint) and the contributions (mechanical and secretory)
from the adjacent skeletal musculature. Previous
analysis of Myf5-/-
:MyoD-/- mouse fetuses lacking
skeletal muscle demonstrated the importance of muscle
contraction and static loading in mouse skeletogenesis.
Among abnormal skeletal features, micrognathia
(mandibular hypoplasia) was detected: small, bent and
posteriorly displaced mandible. As an example of
Waddingtonian epigenetics, we suggest that muscle, in
addition to acting via mechanochemical signal
transduction pathways, networks and promoters, also
exerts secretory stimuli on skeleton. Our goal is to
identify candidate molecules at that muscle-mandible
interface. By employing Systematic Subtractive
Microarray Analysis approach, we compared gene
expression between mandibles of amyogenic and wild
type mouse fetuses and we identified up- and downregulated genes. This step was followed by a
bioinformatics approach and consultation of webaccessible mouse databases. We searched for individual
tissue-specific gene expression and distribution, and for
the functional effects of mutations in a particular gene.
The database search tools allowed us to generate a set of
candidate genes with involvement in mandibular
development: Cacna1s, Ckm, Des, Mir300, Myog and
Tnnc1. We also performed mouse-to-human translational
experiments and found analogies. In the light of our
findings we discuss various players in mandibular
morphogenesis and make an argument for the need to
consider mandibular development as a consequence of
reciprocal epigenetic interactions of both skeletal and
non-skeletal compartments.
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