Publication: Alterations in liver parenchyma after sialoadenectomy in mice, Contribution of neutrophils and macrophages to the removal of damaged hepatocytes
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Date
2008
Authors
Viladrich, Meritxel ; Sánchez, Olga ; Soley, María ; Ramírez, Ignasi
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Surgical excision of submandibular salivary
glands (sialoadenectomy) alters cell turnover in mice
liver. Here we show that the liver of adult mice
contained scattered leukocyte infiltration foci whose size
was in the range of the diameter of hepatocytes. The
number of infiltration foci in the liver increased soon
after sialoadenectomy and remained high for several
weeks. Neutrophils were recruited on dying hepatocytes
soon after the initiation of the apoptotic process. Kupffer
cells appeared later in the process. Just 2 days after
sialoadenectomy, the number of type I infiltration foci
(corresponding to the first stage) had increased 5-fold.
Since these alterations in liver structure are coincident
with a transient decrease in plasma EGF concentration,
we studied whether inhibition of EGF receptor by means
of genistein injection produced a similar effect. After
three days of genistein administration, the number of
type I infiltration foci increased 3.5-fold.
Sialoadenectomized mice were more susceptible than
controls to endotoxin shock. While 90% of shamoperated
mice survived a burst of 100 μ g
lipopolysaccharide/Kg (combined with D-galactosamine
750 mg/Kg), only 50% of sialoadenectomized mice survived. The surviving sialoadenectomized mice
recovered more slowly than the controls, as indicated by
the high plasma alanine transaminase activity a week
after the burst. We conclude that (i) neutrophils and
macrophages participate in the process of apoptotic
hepatocyte removal in a sequential manner; (ii) although
the alteration of liver structure induced by
sialoadenectomy is mild, it has delayed consequences on
the ability of the liver to deal with aggressive insults.
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