Publication: NovoMem® vs CorMatrix®: Decellularisation efficiency and extracellular matrix integrity for future vascular grafting
Authors
Jun Wei Heng ; Shalini Rajan ; Nur Sabrina Zainal Abidin ; Ng Min Hwei1 ; Nadiah Sulaiman ; Ahmad Hafiz Murtadha
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Publisher
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Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-25-009
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info:eu-repo/semantics/article
Description
Abstract
NovoMem®, a collagen membrane derived
from supercritical carbon dioxide (scCO2) decellularised
porcine pericardium, is currently used as a barrier in
dental bone grafts. In line with sustainable development
goals, repurposing NovoMem® for vascular grafts
presents a strategic opportunity. This study aims to
evaluate NovoMem®'s decellularisation efficiency and
extracellular matrix (ECM) preservation to assess its
potential for vascular tissue engineering. The
decellularisation efficiency was assessed using
haematoxylin and eosin (H&E) staining and DNA
quantification to confirm cellular removal and fulfilment
of the proposed minimal criteria of decellularisation.
ECM integrity was evaluated through collagen staining
(Picrosirius red), elastin staining (Elastin van Gieson),
and an insoluble collagen assay to measure total
collagen. NovoMem® showed significantly reduced
cellular content while preserving ECM architecture. The
decellularised tissue had minimal residual DNA and
retained its collagen framework. Compared with
CorMatrix®, a commercially available chemically
decellularised cardiac patch from porcine small intestinal
submucosa (SIS) that has been repurposed for vascular
grafts, NovoMem® exhibited superior decellularisation
efficiency with comparable ECM preservation.
NovoMem® also possesses biocompatibility, supporting
mesenchymal stem cell growth. In conclusion,
NovoMem® has minimal cellular content with preserved
structural integrity, thus suggesting it as an effective
vascular graft that could integrate with host tissues with minimal risk of alloreactivity, potentially improving
graft efficacy and long-term patency.
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