Publication: Human skin reconstruct models: A new application for studies of melanocyte and melanoma biology
Authors
Berking, C. ; Herlyn, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Studies of melanocyte and melanoma
biology using monocultures of cells are limited because
of culture-induced morphology changes and expression
of genes related to growth, migration, and invasion,
which do not reflect the in situ phenotype of normal
melanocytes, nevus cells, or melanoma cells from
biologically early progression stages. The development
of organotypic cultures of human skin, in which culture
artifacts are greatly diminished and cell-matrix and cellcell
interactions between different cell types can be
investigated in a three-dimensional system, has opened a
new era for melanoma research. Long-term in vivo
studies, especially important for melanomagenesis and
melanoma metastasis have become possible through
grafting of skin reconstructs to immuno.deficient
laboratory animals. In this review, principles and
different methods of skin reconstruction are introduced
with focus on the application for pigment cell biology
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Citation
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