Publication: Histological differences in healing following experimental transmural infarction in rats
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Date
2010
Authors
Aljinović, Jure ; Vukojevic, Katarina ; Košta, Vana ; Marinović Guić, Maja ; Saraga-Babić, Mirna ; Grković, Ivica
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Mechanisms of cardiac regeneration
following transmural myocardial infarction were
analysed in rat hearts using immunohistochemistry for
α-SMA, caspase-3, Ki-67 and nestin markers. Seven
weeks after experimental myocardial infarction, two
different types of healing processes were revealed in rats
with and without aneurysmatic bulging of the left
ventricular wall.
Besides thinning of the ventricular wall, three zones
characterized both types of scars: the scar zone (divided
into central and peripheral parts), the peri-infarct zone
and the border zone. The main difference between the
types of scars was the presence of a central necrotic zone
inside the aneurysmatic wall, while connective tissue
with myofibroblasts characterized the same zone in nonbulging
wall. Apoptotic caspase-3 positive cells were
found in the granulation tissue of the border zone in
aneurysmatic scar, while in non-bulging scar they
characterized all three zones. Proliferating Ki-67
positive cells displayed reverse expression pattern
compared to apoptotic cells. Quantification of α-SMA
positive cells revealed 60% α-SMA positive cells inside
the central part of the aneurysmatic scar zone and 39%
in invaginating areas, versus 19% in non-invaginating
areas of the peripheral zone, but only 30% in the
peripheral part of the non-bulging scar zone. Nestin
positive cells were found in both types of scars, but with
different distribution. These results suggest that even
seven weeks after myocardial infarction, the healing
processes in non-bulging scars are in chronic phase,
while aneurysmatic scars are still in subacute phase.
Histological differences in scar healing might be
important for functional properties of the heart wall and
for heart recovery prognosis.
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