Publication: Assessment of immunologic, proangiogenic and neurogenic properties of human peripheral nerve epineurium for potential clinical application
Authors
Klimczak, Aleksandra ; Siemionow, Maria ; Futoma, Katarzyna ; Jundzill, Arkadiusz ; Patrzalek, Dariusz
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-875
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info:eu-repo/semantics/article
Description
Abstract
The epineural sheath is a promising naturally
occurring material for enhancement of peripheral nerve
regeneration. Based on a literature search there is a
limited number of reports on the biological and
immunological properties of human epineurium. The
goal of this study was to assess, using immunocytochemical methods, the immunological (HLA class I and
II antigens, T lymphocytes, macrophages), proangiogenic (VEGF, CD31), and neurogenic (GFAP, S-100)
properties of human epineurium isolated from
ilioinguinal nerves (n=19) taken from deceased donors,
and from sciatic nerves (n=12) taken from limbs
amputated due to critical ischemia. Our studies
confirmed reduced expression of HLA class II antigens
on the infiltrating cells, a reduced number of T
lymphocytes, and greater vessel density in the
epineurium obtained from deceased organ donors.
Macrophages were more abundant in the epineurium
isolated from the amputated limbs. We found that the
epineurium harvested from peripheral nerves of the
deceased donors showed negligible immunogenic and
increased proangiogenic properties compared to the
epineurium of nerves taken from amputated limbs. These
findings support the rationale to use human epineurium
obtained from deceased donors as a new biological
material for enhancement of peripheral nerve repair for
potential clinical application in regenerative medicine.
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