Publication: Calbindin D-28k immunoreactive nerve fibers in the carotid body of normoxic and chronically hypoxic rats
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Date
2000
Authors
Kusakabe, T. ; Matsuda, H. ; Hirakawa, H. ; Hayashida, Y. ; Ichikawa, T. ; Kawakami, T. ; Takenaka, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The distribution and ultrastructural
characteristics of calbindin D-28k immunoreactive nerve
fibers were examined in the carotid body of the
normoxic control rats by light and electron microscopy,
and the abundance of calbindin D-28k fibers in the
carotid body was compared in normoxic and chronically
hypoxic rats (10% O2 and 3.0-4.0% CO2 for 3 months).
Calbindin D-28k immunoreactivity was recognized in
nerve fibers within the carotid body. Calbindin D-28k
immunoreactive nerve fibers appeared as thin processes
with many varicosities. They were distributed around
clusters of glomus cells, and around blood vessels.
Immunoelectron microscopy revealed that the calbindin
D-28k immunoreactive nerve terminals are in close
apposition with the glomus cells, and membrane
specialization is visible in some terminals. Some densecored
vesicles in the glomus cells were aggregated in
this contact region. The chronically hypoxic carotid
bodies were found to be enlarged several fold, and a
relative abundance of calbindin D-28k fibers was lesser
than in the normoxic carotid bodies. When expressed by
the density of varicosities per unit area of the
parenchyma, the density of calbindin D-28k fibers
associated with the glomus cells in chronically hypoxic
carotid bodies was decreased by 70%. These
immunohistochemical findings indicate a morphological
basis for involvement of calcium binding protein in the
neural pathway that modulates carotid body
chemoreception.
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