Publication: Exosomes derived from endothelial progenitor cells ameliorate glyoxylate deprivation (OGD)-induced neuronal apoptosis by delivering miR-221-3p
Authors
Pan, Jie ; Wu, Tingting ; Chen, Bo ; Wu, Huadong
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-18-528
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info:eu-repo/semantics/article
Description
Abstract
This study evaluated the potential of
endothelial progenitor cell (EPC)-derived exosomes as a
therapeutic factor for neuronal apoptosis. Mouse EPCs
were cultured in vitro, and exosomes were isolated and
identified using transmission electron microscopy
(TEM), particle size analysis and by determining the
protein expressions of exosome markers (CD9, CD63
and Alix). The apoptotic rate of OGD-treated neurons
was detected by Flow cytometry assay. The mRNA and
protein expression levels were detected by RT-PCR and
Western blot assay, respectively. Luciferase reporter
assays determined the interaction between miR-221-3p
and Bcl2l11. The results showed that most exosomes are
80-120 nm in diameter. Western blot assay showed that
CD9, CD63 and Alix were enriched in exosomes. EPCderived exosomes ameliorated OGD-induced neuronal
apoptosis. Mechanistically, miR-221-3p from EPCderived exosomes decreased the expression of bcl2l11 in
OGD-induced neuronal apoptosis. Moreover, exosomes
from miR-221-3p mimics transfected EPCs reduced
OGD-induced neuronal apoptosis. In conclusion, miR221-3p in EPC derived exosomes ameliorates OGDinduced neuronal apoptosis, which establish its potential
as a new therapeutic method for patients with
cerebrovascular diseases.
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Citation
Histology and Histopathology, Vol.38, nº4, (2023)
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