Publication: Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system
Authors
Soares, C.P. ; Zuanon, J.A.S. ; Teresa, D.B. ; Fregonezi, P.A. ; Neto, C.B. ; Oliveira, M.R.B. ; Donadi, E.A. ; Martinelli-Kläy, C.P. ; Soares, E.G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The knowledge of cell-cycle control has
shown that the capacity of malignant growth is acquired
by the stepwise accumulation of defects in specific genes
regulating cell growth. Histologic diagnosis might be
improved by a quantitative evaluation of more specific
diagnosis biomarkers, which could help to precisely
identify pre-malignant and malignant oral lesions. The
aim of the present study is to evaluate whether
computer-based quantitative assessment of p53, PCNA
and Ki-67 immunohistochemical expression, could be
used clinically to foresee the risk of oral malignant
transformation. This retrospective study was carried out
in ninety-five oral biopsies, 27 were classified as fibrous
inflammatory hyperplasia, 40 as leukoplakia and 28 as
oral squamous cell carcinoma. Sixteen out of the 40
leukoplakia were diagnosed as non-dysplastic
leukoplakia, the other 24 being dysplastic leukoplakia,
of which 50.0% were classified as moderate to severe
dysplasia. Comparison of the four groups of oral tissues
showed significant rises in p53 and Ki-67 positivity
index, which increased steadily in the order benign, premalignant,
and malignant. In contrast, it was not possible
to relate higher PCNA levels with pre-malignant and
malignant oral lesions. We therefore conclude that PCNA immunohistochemistry expression is probably an
inappropriate marker to identify oral carcinogenesis,
whereas joint quantitative evaluation of p53 and Ki-67,
appears to be useful as a tumor marker, providing a prediagnostic
estimate of the potential for cell-cycle
deregulation of the oral proliferate status.
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