Publication: MiR-194-3p modulates the progression of colorectal cancer by targeting KLK10
Authors
Liu, Tao ; Fang, Yuejun
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-413
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info:eu-repo/semantics/article
Description
Abstract
Background. A rich history of studies have
manifested the importance of miRNAs to cancer
progression, while miR-194-3p has been seldom
explored.
Objective. The purpose of this study is to unearth the
way the miR-194-3p/KLK10 axis modulates colorectal
cancer (CRC).
Methods. Differentially expressed genes of CRC in
TCGA database were analyzed. Western blot and qRTPCR were employed to test protein and mRNA
expressions of two researched genes. Their targeting was
confirmed using dual-luciferase. Biological behaviors of
cells were tested by a series of cellular functional assays.
Result. Remarkably low miR-194-3p expression and
high KLK10 expression were observed in cancer cells.
Overexpressing miR-194-3p hindered the progression of
CRC cells. Overexpression of miR-194-3p significantly
weakened the promoting effect of upregulated KLK10
on cell migration, invasion and proliferation. Their
targeting was verified by dual -luciferase assay.
Therefore, miR-194-3p hindered cell behaviors of CRC
through KLK10.
Conclusion. This investigation casts new light on the
treatment of CRC through the miR-194-3p/KLK10 axis.
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Citation
Histology and Histopathology Vol. 37, nĀŗ3 (2022)
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Este Ćtem estĆ” sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/