Publication: Does diabetic state affect CO-localization of peptide W and enteroglucagon in colonic endocrine cells?
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Date
2000
Authors
Spangeus, A. ; Forsgren, Sture ; El-Salhy, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Background: Changes in the numbers of
PYY- and enteroglucagon-immunoreactive cells in colon
of animal models of human diabetes have been reported.
As these peptides CO-localize in the same cells it is
possible that the observed changes are a result of
changes in CO-localization. Methods: Animal models of
human type 1 and type 2 diabetes, namely the non-obese
diabetic (NOD) mouse and the obese (oblob) mouse,
were studied. As controls for the NOD mice, BALBIcJ
mice were used and for oblob mice, homozygous lean
(+l+) mice were used. Tissue samples from colon were
double-immunostained for PYY and enteroglucagon
according to the indirect immunofluorescence method.
Results: CO-localization of enteroglucagon and PYY was
found in colonic endocrine cells in all groups
investigated. Compared with controls, pre-diabetic NOD
mice showed a decreased proportion of enteroglucagon1PYY
CO-localization. There was no difference
in diabetic NOD mice or diabetic oblob mice when
compared with controls. Conclusions: Whereas the
number of cells containing solely enteroglucagon and
solely PYY increases in pre-diabetic NOD mice,
production of enteroglucagon in PYY-immunoreactive
cells decreases. Although the numbers of PYY and
enteroglucagon cells have been reported to be changed
in both diabetic NOD mice and in obese mice, the
balance between CO-expressing and mono-expressing
cells seems to be preserved.
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