Publication: Flutamide alters β-catenin expression and distribution, and its interactions with E-cadherin in the porcine corpus luteum of mid- and late pregnancy
Authors
Grzesiak, Malgorzata ; Mitan, Agata ; Janik, Marcelina E. ; Knapczyk-Stwora, Katarzyna ; Slomczynska, Maria
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
10.14670/HH-11-630
item.page.type
info:eu-repo/semantics/article
Description
Abstract
This study examined whether flutamideinduced androgen deficiency during mid- and late
pregnancy in pigs affected luteal expression of adherens
junction protein, β-catenin, and its interactions with Ecadherin. Flutamide (50 mg/kg body weight) was
administered into pregnant gilts between days 43-49
(GD50F), 83-89 (GD90F) or 101-107 (GD108F) of
gestation. Corpora lutea (CLs) were obtained on day 50,
90 or 108 of pregnancy (n=8-11 per each group). Total
β-catenin and E-cadherin expression was examined at
mRNA (real-time PCR) and protein (Western blot) level.
Moreover, subcellular β-catenin fractions were extracted
and immunoblotted. Immunohistochemistry was used for
β-catenin localization. To determine whether flutamide
disturbs β-catenin/E-cadherin mutual interactions,
coimmunoprecipitation using anti-β-catenin antibody
was performed. Furthermore, phosphorylation of Ecadherin was assessed. Flutamide exposure led to
decreased β-catenin mRNA expression in all examined
groups (p<0.001 or p<0.01), but protein level was lower
only in the GD90F and GD108F groups (p<0.05). Ecadherin mRNA (p<0.05 or p<0.01) and protein
(p<0.05) levels were up-regulated in all flutamidetreated groups when compared to controls. β-catenin was
predominantly found in membranes of luteal cells with
no significant changes after antiandrogen treatment. βcatenin/E-cadherin complexes were more abundant in
the GD90F (p<0.05) and GD108F (p<0.01) groups than
in controls due to enhanced E-cadherin phosphorylation
at serine 838/840 in those animals (p<0.05). Overall,
although androgen deficiency affected β-catenin
expression in the CL of pregnancy in pigs, a
compensatory mechanism by enhanced interactions with
E-cadherin is possible. Thus, androgen signaling via
androgen receptors appears to be crucial in the
regulation of luteal cells cross-talk.
publication.page.subject
Citation
Histology and Histopathology, vol. 30, nº11, (2015)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/3.0/es/