Publication: Characterization and mRNA expression in an unusual odontogenic lesion in a patient
with tricho-dento-osseous syndrome
Authors
Dodds, A.P. ; Cox, S.A. ; Suggs, C.A. ; Boyd, C. ; Hart, T.C. ; Wright, J.T.
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Publisher
Murcia : F. Hernández
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Ruiz, R.
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Odontogenic lesions are rare, but can be
associated with significant morbidity. While their
molecular determinants are unknown, they likely express
many genes common to normal odontogenesis. This
study evaluated the histology and mRNA expression of
an unusual odontogenic lesion in a patient with a
confirmed history of tricho-dento-osseous syndrome.
Methods: Decalcified, frozen 8 µm sections of the lesion
were cut and mounted on glass slides and stained with
hematoxylin/eosin for analysis. The expression of
multiple genes associated with normal odontogenesis
and related pathologies were evaluated by RT-PCR,
where possible in samples of the hard and soft tissue
components of the lesion. Results: Histological
examination showed the lesion had large areas of
irregular, dentine-like material, enamel matrix, areas of
woven immature bone and multiple fully mineralised
tooth crowns. Although most of the gene transcripts
were amplified from both samples, some, including
DLX3/7 and Collagen I demonstrated differential
expression. Conclusions: This study shows the gene
expression profile of aberrant odontogenesis with
associated odontoma formation is similar to that of
normal tooth and the genes expressed in other
odontogenic lesions. While the role of altered gene
expression in the development of such lesions has
previously been postulated from transgenic models, this
is the only report of an odontogenic lesion in a patient
with TDO, and begins to elucidate possible gene
interactions key to its development.
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