Publication: Novel polyvinyl alcohol (PVA) based cryoprotection method that facilitates cutting
frozen sections of decalcified human trabecular bone
Authors
Maly, I. Piotr ; Rahner, Christoph ; Nowakowski, Andrej Maria ; Müller-Gerbl, Magdalena
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Processing adult human trabecular bone to
obtain tissue sections suitable for research or diagnostic
purposes has always been challenging, particularly in the
preparation of adult bone specimens for advanced
immunohistochemistry applications. In contrast to the
majority of soft tissues, decalcified bone samples
perform poorly under standard paraffin embedding
techniques and immunolabeling protocols fail frequently,
due to the loss of protein antigenicity observed. We
report on a new, PVA based infiltration method that
avoids excessive heat exposure to tissue samples during
embedding. The developed PVA based infiltration
medium provides sufficient structural support to the
heterogenic morphology and distinct architecture of
subchondral trabecular bone and adjacent articular
cartilage. Furthermore, the addition of bovine serum
albumin (BSA) to this infiltration solution guaranteed
safe attachment of cryosections to glass slides. The
protocol allows the preparation of high quality sections
of adult human trabecular bone tissues which can be
used for both classical histochemical stains and for
immunohistochemistry, since protein antigenicity is
satisfactorily preserved.
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Citation
Histology and Histopathology, vol. 28, nº 12 (2013)
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