Publication: CircRIP2 promotes NSCLC progression by sponging for miR-671-5p to regulate FOXM1 expression
Authors
Liu, Yizhi ; Feng, Xing ; Kang, Shuhong ; Lv, Feng ; Ni, Yunfeng ; Wu, Hua
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-360
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info:eu-repo/semantics/article
Description
Abstract
Lot of attention had been paid to the role of
circular RNAs (circRNAs) in carcinogenesis recently.
However, knowledge about circRNAs in NSCLC
development is far from satisfactory. In this study, we
aimed to provide a novel insight into the circRIP2 in
NSCLC development. We used NSCLC tissues, as well
as cell lines to elucidate the expression and location of
circRIP2 in NSCLC. We also established the circRIP2
overexpression cells A549-circRIP2 and repression cells
HCC827-shcircRIP2 for further functional and
mechanism studies. The pro-tumorigenic role of
circRIP2 was tested by using CCK-8, BrdU and
transwell assays. The interaction between circRIP2 and
miR-671-5p were validated by luciferase reporter assay,
RIP assay, as well as RNA pull down assay. We showed
circRIP2 is differentially expressed NSCLC, and acted
as a predictor for overall survival (OS) and disease-free
survival (DFS). CircRIP2 promoted NSCLC progression
by acting as a miRNA sponge for miR-671-5p, thus
facilitating its target gene FOXM1 expression. Targeting
circRIP2 could be potentially beneficial for NSCLC
patients in the future
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Citation
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