Publication: In situ demonstration of both TUNEL-labeled germ cell and Sertoli cell in the cimetidine-treated rats
Authors
Sasso-Cerri, E. ; Miraglia, S.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cimetidine has caused dysfunction in the
male reproductive system. In the rat testis, intratubu l a r
alterations and loss of peritubular tissue due to
p e r i t u bular myoid cell death by apoptosis have been
recently shown. Thus, the aim of this study is to evaluate
which cells of the seminiferous epithelium have been
affected and/or died by apoptosis after the treatment with
cimetidine.
For this purpose, an experimental group containing
five male albino Wistar rats received intraperitoneal
injections of cimetidine (50 mg/kg body weight) during
52 days. The testes were f i xed with 4% bu ff e r e d
f o r m a l d e hyde and were embedded in paraffin. Fo r
detection of DNA breaks (apoptosis) in the cells of the
seminiferous epithelium, the testicular sections were
treated by the TUNEL method (Apop-Tag Plus
Peroxidase Kit).
In the tubules affected by cimetidine, altered
p e r i t u bular tissue, including the presence of TUNEL
labeling in the myoid peritubular cells, were usually
found. In these tubules, the seminiferous epithelium
exhibited low density of germ cells and TUNEL-positive
labeling in the germ cells of the basal compartment. The
concomitant staining in both germ cells of the basal
compartment and late spermatids suggest a sensitivity of
these cells in the damaged tubules. Besides germ cells,
T U N E L - p o s i t ive Sertoli cells were also found in the
injured seminiferous tubules. Thus, a relationship
between dying germ cells and Sertoli cell damage and/or
death must be considered in tubules where peritubu l a r
tissue has been affected by toxicants.
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