Publication: Role of an endothelin type A receptor antagonist in regulating torsion-induced testicular apoptosis in rats
Authors
Cayli, Sevil ; Ocakli, Seda ; Senel, Ufuk ; Karaca, Zafer ; Erdemir, Fikret ; Delibasi, Tuncay
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-698
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info:eu-repo/semantics/article
Description
Abstract
Testicular torsion is a well-known medical
emergency that can lead to pathological changes in the
testicular tissues and male infertility. This investigation
was undertaken to gain insight into the effects of an
endothelin type A receptor antagonist (BQ123) on
torsion-induced germ cell loss. Twenty-eight male
Wistar albino rats were divided into four groups. In
group I (control group), a sham operation to the left
testis was performed. In group II (I/R injury), I/R injury
was created by rotating the left testis 720° in a clockwise
direction for 2 h and detorsing the testis after 2 h. In
group III (I/R injury+BQ123), the rats were subjected to
I/R injury and BQ123 injection (1 mg/kg, intravenous).
In group IV (control+BQ123), the sham operated rats
were subjected to BQ123. The testes of the rats were
removed in all groups. Torsion-induced apoptosis and
the effects of BQ123 were examined by the terminal
deoxynucleotidyl transferase-mediated deoxyuridine
triphosphate (dUTP) nick end labelling (TUNEL)
technique, immunohistochemistry and western blotting.
In group II, the number of TUNEL-positive cells
increased after testicular torsion. Immunohistochemistry
and western blotting showed that apoptotic proteins
(active caspase 3 and Bax) were upregulated, and the
anti-apoptotic protein Bcl2 was downregulated in I/R
injury. The administration of BQ123 caused a significant
decrease in the number of apoptotic cells and the
expression of apoptotic proteins (p<0.05) when
compared with the I/R injury group. No significant effect
of BQ123 was observed in the testicular cells of group
IV. This animal study provides evidence of the
regulatory effects of BQ123 on torsion-induced
testicular apoptosis.
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