Publication: CDC28 protein kinase regulatory subunit 1B (CKS1B)
expression and genetic status analysis in oral squamous
cell carcinoma
Authors
Martín-Ezquerra, Gemma ; Salgado, Rocio ; Toll, Agustí ; Baró, Teresa ; Mojal, Sergi ; Yébenes, Mireia ; Garcia-Muret, María Pilar ; Solé, Francesc ; Alameda Quitllet, Francesc ; Espinet, Blanca ; Pujol, Ramón M.
item.page.secondaryauthor
item.page.director
Publisher
Murcia: Universidad de Murcia, Facultad de Biología
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
CKS1B is a member of the highly conserved
cyclin kinase subunit 1 (CKS1) protein family which
interacts with cyclin-dependent kinases and plays a
critical role in cell cycle progression. In oral squamous
cell carcinoma (OSCC), as in other malignancies,
CKS1B overexpression has been correlated with reduced
survival. To our knowledge, no studies evaluating the
genetic status of CKS1B gene in OSCC have been
reported. Herein, genetic and protein status of CKS1B
were analyzed by immunohistochemical (IHC) and
fluorescence in situ hybridization (FISH) techniques in a
series of primary OSCC (n=51) and lymph node OSCC
metastases samples (n=14). The observed results were
compared with those obtained in either inflammatory
(oral lichen planus [OLP]) (n=13) and premalignant oral
mucosal lesions (oral leukoplakia) (n=16). A significant
CKS1B overexpression was observed in OSCC and
lymph node metastases samples than in OLP and oral
leukoplakia (mean 70% vs 35%, p<0.001). CKS1B
overexpression correlated with p27 loss of expression
(p=0.0013) and SKP2 overexpression (p<0.00). FISH
study disclosed statistical differences in both gene
amplifications and gains between samples corresponding
to OSCC and metastases from those of OLP and
leukoplakia (p<0.001). Amplifications were present in
53% of OSCC samples and 33% of lymph node
metastases vs 14% of oral leukoplakia and 0% of
OLP biopsy specimens (p=0.002). Polysomies of chromosome 1 were seen in 46% of OSCC, 33% of
ganglionar metastases, 14% of oral leukoplakia and 10%
of OLP (p=0.036). Correlation of CKS1B overexpression
and gains (both polysomies and
amplifications) determined by FISH was statistically
significant (p<0.001).
Our results indicate that a high CKS1B expression is
a common finding in primary OSCC which correlates
with p27 low expression and SKP2 overexpression. This
phenomenon may be due either to numerical
(chromosome 1 polysomy) or structural (amplifications)
CKS1B genetic abnormalities. This phenotypical and
cytogenetic profile is not observed in premalignant or
inflammatory oral mucosal lesions.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.