Publication: MiR-222-3p promotes the proliferation, migration and invasion of papillary thyroid carcinoma cells through targeting SLC4A4
Authors
Zhang, Chunying ; Chang, Qing ; Hu, Yaojie ; Chang, Wang ; Guo, Xin ; Fu, Liru ; Tang, Guoshuai ; Chen, Chunyou
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-387
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info:eu-repo/semantics/article
Description
Abstract
Objective. An increasing number of studies
indicate that miR-222-3p is upregulated in various
cancers and can regulate tumor progression. This study
aimed to explore the regulatory mechanism of miR-222-
3p in papillary thyroid carcinoma (PTC).
Methods. TCGA database was used to dig
differentially expressed miRNAs and mRNAs in PTC
tissue. Relevant references were searched to determine
target miRNA. StarBase, TargetScan and miRDB were
applied to predict mRNAs that had binding sites with the
target miRNA. Then, the mRNAs were intersected with
differentially downregulated mRNAs in TCGA to
determine the target mRNA. qRT-PCR was exerted to
evaluate gene expression of miR-222-3p and SLC4A4 in
PTC. Western blot was performed out to evaluate the
protein expression of SLC4A4 in PTC cells. CCK-8,
wound healing assay and cell invasion assay were
undertaken to observe the proliferative, migratory, and
invasive abilities of PTC cells. Dual-luciferase assay was
employed to test the binding relationship between miR222-3p and SLC4A4.
Results. MiR-222-3p was highly expressed in PTC
while SLC4A4 was lowly expressed. Moreover, miR222-3p was able to promote the proliferation, invasion,
and migration of PTC cells. SLC4A4 was able to reverse
these promotive effects of miR-222-3p.
Conclusion. MiR-222-3p can promote the
proliferation, migration and invasion of PTC cells
through targeting SLC4A4. MiR-222-3p is expected to
be a molecular therapeutic target for PTC patients.
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Citation
Histology and Histopathology Vol. 36, nĀŗ11 (2021)
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Este Ćtem estĆ” sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/