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Proteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertension

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dc.contributor.authorOhata, Yuichiro
dc.contributor.authorOgata, Sho
dc.contributor.authorNakanishi, Kuniaki
dc.contributor.authorKanazawa, Fumiko
dc.contributor.authorUenoyama, Maki
dc.contributor.authorHiroi, Sadayuki
dc.contributor.authorTominaga, Susumu
dc.contributor.authorToda, Tosifusa
dc.contributor.authorKawai, Toshiaki
dc.date.accessioned2018-06-25T16:21:37Z
dc.date.available2018-06-25T16:21:37Z
dc.date.issued2013
dc.description.abstractExperimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung. Proteomics - which may be the most powerful way to uncover unknown remodeling proteins involved in enhancing cardiovascular performance - was used to study 150 male Wistar rats housed for up to 21 days in a chamber at the equivalent of 5500 m altitude level. After 14 days’ exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided according to their hypobaric period) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30 kDa-100 kDa). In hypobaric rats, three spots were increased two-fold or more (vs. control rats) in two-dimensional differential in-gel electrophoresis (2D-DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of heat shock protein 70 (HSP70) and two isoforms of protein disulfide isomerase associated 3. This result was confirmed by Western blotting analysis of 2D-PAGE. Conceivably, HSP70 and PDIA3 may play roles in modulating the lung structural remodeling that occurs due to pulmonary hypertension in hypobaric hypoxia.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology, vol. 28, nº 7 (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/59620
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectProteomicses
dc.subjectHypobaric hypoxiaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleProteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertensiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 7 (2013)