Publication: Histology of skeletal muscle reconstructed by means of the implantation of autologous adipose tissue: an experimental study
Authors
Leiva Cepas, Fernando ; Jimena, Ignacio ; Ruz Caracuel, Ignacio ; Luque, Evelio ; Villalba, Rafael ; Peña Amaro, Jose
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org10.14670/HH-18-163
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to determine
the histological characteristics of a skeletal muscle
reconstructed by means of the implantation of
autologous adipose tissue following an experimentally-
induced volumetric muscle loss. A cylindrical piece in
the belly of the rat anterior tibial muscle was removed.
In the hole, inguinal subcutaneous adipose tissue of the
same rat was grafted. Animals were sacrificed 7, 14, 21,
28 and 60 days posttransplantation. Histological,
histochemical, immunohistochemical and morphometric
techniques were used. At all times analyzed, the
regenerative muscle fibers formed from the edges of the
muscle tissue showed histological, histochemical and
immunohistochemical differences in comparison with
the control group. These differences are related to delays
in the maturation process and are related to problems in
reinnervation and disorientation of muscle fibers. The
stains for MyoD and desmin showed that some
myoblasts and myotubes seem to derive from the
transplanted adipose tissue. After 60 days, the transplant
area was 20% occupied by fibrosis and by 80% skeletal
muscle. However, the neo-muscle was chaotically
organized showing muscle fiber disorientation and
centronucleated fibers with irregular shape and size. Our
results support the hypothesis that, at least from a
morphological point of view, autologous adipose tissue
transplantation favors reconstruction following a
volumetric loss of skeletal muscle by combining the
inherent regenerative response of the organ itself and the
myogenic differentiation of the stem cells present in the
adipose tissue. However, in our study, the formed neo-
muscle exhibited histological differences in comparison
with the normal skeletal muscle
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