Publication: Expression of the GABAA receptor/Chloride
Channel in murine spermatogenic cells
Authors
Kanbara, Kiyoto ; Mori, Yoshiaki ; Kubota, Takahiro ; Watanabe, Masahito ; Yanagawa, Yuchio ; Otsuki, Yoshinori
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Previous studies from our laboratory have
demonstrated that γ-aminobutyric acid (GABA) and
GABAB receptor subunits are expressed within the
acrosome of spermatids during spermiogenesis.
Furthermore, our previous study with the glutamate
decarboxylase (GAD) 67-GFP knock-in mouse
demonstrated that GFP–positive cells were localized to
the epithelium of the caput of epididymis. In the present
study, we detected GABAA subunits, including α1, α5,
ß1-3 and γ3, and both isoforms of GAD, GAD65 and
GAD67, in mouse spermatogenic cells using RT-PCR.
The expression of these proteins was subsequently
confirmed by western blot analysis. Immunohistochemistry
also revealed that GABA, GAD65, and α5, ß1
and γ3 subunits of the GABAA receptor were localized
in the membrane of spermatogenic cells, including
spermatocytes and spermatids. The whole-cell patchclamp
analysis demonstrated that GABA application
induced an inward chloride current in some of the large
and round spermatogenic cells. Our findings show that
spermatogenic cells have a GABA producing system by
themselves, and that GABA may function via the
ionotropic GABAA receptor. This data suggests that the
GABAergic system may play important roles in the male
reproductive system.
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