Publication:
Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells

Simple item page
dc.contributor.authorDíaz-Gil, Juan J.es
dc.contributor.authorGarcía-Monzón, Carmeloes
dc.contributor.authorRúa, Carmen
dc.contributor.authorMartín Sanz, Paloma
dc.contributor.authorCereceda, Rosa M.
dc.contributor.authorMiquilena-Colina, María E.
dc.contributor.authorMachín, Celia
dc.contributor.authorFernández Martínez, Amalia
dc.contributor.authorGarcía Cañero, Rafael
dc.date.accessioned2013-09-24T11:34:30Z
dc.date.available2013-09-24T11:34:30Z
dc.date.issued2009
dc.description.abstractThe antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its mechanism of antifibrotic action in bile duct-ligated (BDL) rats, with special emphasis on its activity in fibrogenic liver cells. BDL rats received either LGF 9 μg/week for 2 or 3 weeks (BDL+LGF, n=20/group) or saline (BDL+S, n=20/group), at times 0, week 2, or week 5 after operation. Groups were compared in terms of liver a- smooth muscle actin (SMA) content (western blotting and immunohistochemistry), hepatic apoptosis, liver desmin content (western blotting), and serum endothelin-1 (ELISA). LGF produced a marked decrease in liver a-SMA content compared with saline-injected rats, especially evident at longer times (5w and 8w; p<0.05), accompanied by a decrease in hepatic a-SMA+ cells. This decrease was not due to the killing of activated hepatic stellate cells (HSC) or myofibroblasts by LGF, since there was a slight decrease in hepatic apoptosis that was more evident at 2w (p<0.05). Moreover, LGF did not seem to influence HSC proliferation, as shown by measuring liver desmin content. The antifibrotic activity exerted by LGF seems to be closely related to a modulation of the activation state of fibrogenic liver cells (activated HSC and myofibroblasts) in BDL rats.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/36009
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectFibrosises
dc.subjectMyofibroblastses
dc.subject.other576 - Biología celular y subcelular. Citologíaes
dc.titleLiver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Liver growth factor antifibrotic activity in vivo is associated with a.pdf
Size:
1.44 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.24, nº4 (2009)