Publication: Expression of ribosomal protein L4 (rpL4) during neurogenesis and 5-azacytidine, (5AzC)-induced apoptotic process in the rat
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Date
2002
Authors
Ueno, M. ; Nakayama, Hiroyuki ; Kajikawa, S. ; Katayama, K. ; Suzuki, K. ; Doi, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
5-Azacytidine (5AzC) induces neuronal
apoptosis in rat and mouse fetuses. 5AzC also induces
apoptosis in undifferentiated PC12 cells, and ribosomal
protein L4 (rpL4) mRNA expression increases prior to
apoptosis. To clarify the roles of rpL4 during
neurogenesis, we first examined the distribution of rpL4
mRNA in the developing rat brain by in situ
hybridization and RT-PCR, and compared the results to
the distribution of TUNEL- or PCNA-positive cells.
rpL4 mRNA expression was strong in the ventricular
zone (VZ), subventricular zone (SVZ), cortical plate
(CP), cerebral cortex, granule cell layer (GCL),
pyramidal cell layer (Py) and external granular layer
(EGL) during embryonic and early postnatal days, and it
was remarkably weakened thereafter. A lot of PCNApositive
cells were observed in VZ, SVZ, and EGL
during embryonic and early postnatal days, and such
distribution of PCNA-positive cells was almost identical
to rpL4 mRNA distribution. Only few TUNEL-positive
cells were observed in VZ, SVZ, cerebral cortex, EGL,
and hippocampus during embryonic and early postnatal
days, and the regions with TUNEL-positive cells were
not identical to rpL4 mRNA distribution. Next, the
changes of rpL4 mRNA expression in the brain of
5AzC-treated rat fetuses were examined by in situ
hybridization and RT-PCR. Apoptotic cells appeared at 9
to 24 hours after treatment (HAT). However, the rpL4
mRNA expression was unchanged during the apoptotic
process. From the results, it is suggested that rpL4 would
have certain roles in cell proliferation and differentiation
during neurogenesis, but have no roles in 5AzC-induced
apoptosis in the fetal brain.
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