Publication: A putative role for calpain in demyelination associated with optic neuritis
Loading...
Date
1999
Authors
Banik, N.L. ; Shields, D.C.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Calcium activated neutral proteinase
(calpain) is an endopeptidase present in the central
nervous system which degrades myelin proteins. To
examine the role of calpain in demyelination associated
with optic neuritis, immunocytochemical expression of
calpain was evaluated in Lewis rats with experimental
optic neuritis. Calpain expression was increased in
activated microglia, infiltrating macrophages, activated
T cells, and reactive astrocytes in experimental optic
neuritis compared to controls. Calpain activity and
translational expression were also examined by Western
blotting studies measuring the extent of myelin protein
degradation, calpain-specific fodrin proteolysis, axonal
neurofilament degradation, and calpain proenzyme
content. Results showed myelin associated glycoprotein
and 68 kD neurofilament protein levels were
significantly decreased while calpain translational
expression and calpain-autolyzed fodrin levels were
significantly increased in experimental optic neuritis
compared to controls. Thus, increased activity and
translational expression of calpain in optic neuritis may
be integral to the pathogenesis of this disorder.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.