Publication: The novel histological evidence of the blood-spleen barrier in duck (Anas platyrhynchos)
Authors
Sun, Xuejing ; Liu, Enxue ; Wang, Taozhi ; Zhang, Qian ; Yang, Ping ; Ahmed, Nisar ; Zhao, Qiaoya ; Chen, Qiusheng
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-019
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info:eu-repo/semantics/article
Description
Abstract
To identify the existence and composition of
the blood-spleen barrier (BSB) in ducks, the
microanatomical structures of the duck spleen were
investigated by light and transmission electron
microscopy, silver staining, enzymatic histochemistry
and intravenous injection of ink. The endothelial cells of
the sheathed capillaries were cuboidal-shaped and
surrounded by an ellipsoid consisting of reticular cells,
similar to high endothelial venules (HEVs). After ink
injection, carbon particles were initially restricted to the
ellipsoid and later trapped in the periellipsoidal
lymphatic sheaths (PELS), and then transferred to the
periarteriolar lymphatic sheaths (PALS) and splenic
nodules over time. Reticular fibers were primarily
distributed at the basement membrane of the sheathed
capillaries and the periphery of the PELS. Macrophages
were primarily distributed at the border between red pulp
and PELS. These results suggested that the BSB was
present in the ellipsoid and PELS and consisted of the
mechanical barrier composed of endothelial cells of the
sheathed capillaries, reticular cells and reticular fibers
and the biological barrier composed of ellipsoidassociated macrophages. In conclusion, the BSB was
identified in the duck spleen for the first time, including
cuboidal endothelial cells, ellipsoid-associated
macrophages, reticular cells and fibers, and resisting
circulating pathogen invasions. The study of BSB in
ducks provides a theoretical foundation for the structural
composition of the avian immune system.
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