Publication: Expression of EPO and related factors in the liver and kidney of plain and Tibetan sheep
Authors
Yang, Kun ; Zhang, Lan ; Chen, Weiji ; Cheng, Jialu ; Zhao, Xiaomeng ; Zhang, Yiyang ; Li, Rui ; Zhou, Manlin ; Yao, Yifan ; Li, You ; Qiao, Zilin
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-592
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info:eu-repo/semantics/article
Description
Abstract
Erythropoietin (EPO), hypoxia-inducible
factor-1α (HIF-1α), hypoxia-inducible factor-2α (HIF2α), and vascular endothelial growth factor (VEGF) are
key factors in the regulation of hypoxia, and can
transcriptionally activate multiple genes under hypoxic
conditions, thereby initiating large hypoxic stress in the
network. The liver and kidneys are important metabolic
organs of the body. We assessed the expression of EPO,
HIF-1α, HIF-2α, and VEGF in liver and kidney tissues
of plain and Tibetan sheep using hematoxylin and eosin
staining, immunohistochemistry, and RT-qPCR. The
results showed that EPO, HIF-1α, HIF-2α, and VEGF
were expressed in tubular epithelial cells, collecting duct
epithelial cells, mural epithelial cells, and the glomerular
cytoplasm of Tibetan sheep, and their expression was
significantly higher in Tibetan sheep than in plain sheep
(P<0.05). EPO, HIF-1α, HIF-2α, and VEGF are
expressed in hepatocytes, interlobular venous endothelial
cells, and interlobular bile duct epithelial cells. In plain
sheep, positive signals for EPO, HIF-1α, HIF-2α, and
VEGF were localized mainly in interlobular venous
endothelial cells, whereas VEGF and HIF-2α were
negatively expressed in interlobular bile duct epithelial
cells and positively expressed in EPO and HIF-1α. The
differences in EPO, HIF-1α, and HIF-2α in Tibetan
sheep were significantly higher than those in plain sheep
(P<0.001). In the liver and kidney tissues of Tibetan
sheep, EPO was associated with HIF-1α, HIF-2α, and
VEGF (P<0.05). RT-qPCR results showed that EPO was
not expressed, and HIF-1α, HIF-2α, and VEGF were
expressed (P<0.05). The results showed that the
expression of EPO, HIF-1α, HIF-2α, and VEGF in the
kidney and liver of Tibetan sheep was higher than that in
of plain sheep. Therefore, EPO, HIF-1α, HIF-2α, and
VEGF may be involved in the adaptive response of
plateau animals, which provides theoretical clarity to
further explore the adaptive mechanism of plateau
hypoxia in Tibetan sheep.
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