Publication: Role of skeletal muscle in palate development
Authors
Rot, Irena ; Kablar, Boris
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
Description
Abstract
The involvement of skeletal muscle in the
process of palatal development in mammals is an
example of Waddingtonian epigenetics. Our earlier study
showed that the cleft palate develops in the complete
absence of skeletal musculature during embryonic
development in mice. This contrasts with previous
beliefs that tongue obstruction prevents the elevation and
fusion of the palatal shelves. We argue that the complete
absence of mechanical stimuli from the adjacent muscle,
i.e., the lack of both static and dynamic loading, results
in disordered palatogenesis. We further suggest that
proper fusion of the palatal shelves depends not only on
mechanical but also on paracrine contributions from the
muscle. The muscle’s paracrine role in the process of
palatal fusion is achieved through its being a source of
certain secreted and/or circulatory proteins. A cDNA
microarray analysis revealed differentially expressed
genes in the cleft palate of amyogenic mouse fetuses and
suggested candidate molecules with a novel function in
palatogenesis (e.g., Tgfbr2, Bmp7, Trim71, E2f5, Ddx5,
Gfap, Sema3f). In particular, we report on Gdf11 mutant
mouse that has cleft palate, and on several genes whose
distribution is normally restricted to the muscle
(completely absent in our amyogenic mouse model), but
which are found down-regulated in amyogenic mouse
cleft palate. These molecules probably present a subset
of paracrine cues that influence palatogenesis from the
adjacent muscle. Future studies will elucidate the role of
these genes in muscle-palate crosstalk, connecting the
cues produced by the muscle with the cartilage and bone
tissue’s responses to these cues, through various degrees
of cell p
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.