Publication: Immunolocalization of serum proteins in xenografted mouse model of human tumor cells by various cryotechniques
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Date
2009
Authors
Bai, Y. ; Ohno, N. ; Terada, N. ; Saitoh, S. ; Nakazawa, T. ; Nakamura, N. ; Katoh, R. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The transport mechanism of soluble
molecules throughout the interstitial matrix is closely
associated with human tumor behavior in vivo. However,
the examination of soluble components in histological
architectures has been hampered by artifacts caused
during conventional tissue preparation. In this study, the
immunodistribution of intrinsic and extrinsic serum
components in tumor tissues was examined in
xenografted human tumor cells using ‘in vivo
cryotechnique’ (IVCT) and cryobiopsy, where target
tissues are directly cryofixed in vivo. Human lung cancer
cells were subcutaneously injected into the dorsal flank
of nude mice, and paraffin sections and cryosections of
produced tumors were prepared with different methods.
Immunolocalization of serum proteins, including
albumin, immunoglobulin G (IgG) and IgM, as well as
intravenously injected bovine serum albumin (BSA) was
examined. Their immunodistribution was more clearly
observed in the interstitium by both IVCT and
cryobiopsy than conventional methods. IgM was
immunolocalized within blood vessels, whereas albumin
and IgG were observed in the tumor interstitium.
Moreover, intravenously injected bovine serum albumin
exhibited leakage from the blood capillaries into
surrounding connective tissues in 24 h, but it gradually
diffused to the interstitium of the tumor masses during 3
days. These results suggest that molecular leakage from
blood capillaries varies significantly in different areas of
developing tumors, and that small serum proteins, but
not large ones, were abundantly immunolocalized in the
tumor interstitium. Both IVCT and cryobiopsy were
found to be useful for immunohistochemical studies of soluble molecules in tumors with blood circulation, and
may therefore be helpful for further histopathological
analyses.
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