Publication: Amelioration of hypercholesterolemiainduced hepatic changes with red
grape juice: A histopathological study
Authors
Al-Ahmadi, Ahlam Abdulaziz ; Ali, Soad Shaker ; Ayuob, Nasra Naeim ; Al Ansary, Abeer Khaled
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objectives: Hypercholesterolemia was
confirmed as a risk factor for hepatic fibrosis, as well
atherosclerosis and coronary heart disease. This
biochemical and histoplathological study was conducted
to investigate the possible protective effect of red grape
against hepatic injury induced by a high-cholesterol diet
(HCD).
Material and methods: Thirty male Wister rats were
randomly divided into three groups (n=10): the control
received saline, the induction group was fed HCD, and
the treated group was fed a HCD and 0.4 ml of 100% red
grape juice (RGJ) for 13 weeks. After the animals were
sacrificed, liver tissue samples were taken to be
processed for light and electron microscopy
examination.
Results: The administration of the RGJ and HCD
significantly decreased the animals’ blood glucose,
insulin, cholesterol, triglycerides, Low Density
Lipoprotein levels and increased their High Density
Lipoprotein level compared to the rats fed the HCD
alone. It also decreased the periportal (macro- and
microvesicular) steatosis, fibrosis, lymphocytic
infiltration and blood sinusoidal congestion that were
observed in HCD-fed rats alone. The RGJ reduced the
number of activated myofibrobasts. This was confirmed
by a reduction in the expression of alpha smooth muscle
actin and desmin. The RGJ increased, although not
significantly, the expression of endothelial Nitric Oxide
Synthetase.
Conclusion: The administration of RGJ succeeded in
alleviating the biochemical and, to some extent, the
histopathological changes induced by the high
cholesterol diet. Consumption of fresh RGJ or its
pharmaceutical preparations is advised especially for
those who are used to eat a high fat diet.
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